METABOLIC CHARACTERISTICS OF FIBER TYPES IN HUMAN SKELETAL-MUSCLE
METABOLIC CHARACTERISTICS OF FIBER TYPES IN HUMAN SKELETAL-MUSCLE
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DOI：
10.1111/j.1748-1716.1975.tb10038.x
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
SALTIN, B
中科院分区：
文献类型：
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作者：
ESSEN, B;JANSSON, E;SALTIN, B
为了评估人体骨骼肌纤维类型之间底物（糖原和甘油三酯）、离子浓度（Na+和K+）以及酶活性水平（琥珀酸脱氢酶，SDH；磷酸果糖激酶，PFK； 3 -羟基酰基-辅酶a脱氢酶，HAD；肌球蛋白atp酶）的定量差异，从健康受试者身上获得了肌肉活检样本。冷冻干燥肌肉标本后，将单个纤维的碎片分离出来，在pH值为10.3、4.6和4.35的预孵卵条件下进行肌纤维- atp酶染色。然后可以识别I型（“红色”）和II型A、B和C型（“白色”）纤维。糖原含量在不同纤维中相同，而甘油三酯含量在I型纤维中最高（2-3 × II型）。不同纤维类型间Na+和K+含量无显著差异。不同纤维类型（SDH和HAD， Type I 1.5 × Type II; PFK Type I 0.5 × Type II; Myosin ATPase Type I 0.4 × Type II）的酶活性差异很大。II型纤维亚群通过SDH和PFK活性的差异来区分（SDH, Type II C > A > B； PFK, Type II B > A C）。结论是，人类骨骼纤维的收缩和代谢特性与许多其他物种非常相似。然而，有一个区别似乎是，没有II型纤维的氧化电位高于I型纤维。
Muscle biopsy samples were obtained from healthy subjects in order to evaluate quantitative differences in single fibres of substrate (glycogen and triglyceride) and ion concentrations (Na+and K+) as well as enzyme activity levels (succinate‐dehydrogenase, SDH; phosphofructokinase, PFK; 3‐hydroxyacyl‐CoA‐dehydrogenase, HAD; myosin ATPase) between human skeletal muscle fibre types. After freeze drying of the muscle specimen fragments of single fibres were dissected out and stained for myofibrillar‐ATPase with preincubations at pH's of 10.3, 4.6, and 4.35. Type I (“red”) and II A, B, and C (“white”) fibres could then be identified. Glycogen content was the same in different fibres, whereas triglyceride content was highest in Type I fibres (2–3 × Type II). No significant differences were observed for Na+and K+between fibre types. The activity for the enzymes studied were quite different in the fibre types (SDH and HAD, Type I ≊ 1.5 × Type II; PFK Type I ≊ 0.5 × Type II; Myosin ATPase Type I ≊ 0.4 × Type II). The subgroups of Type II fibres were distinguished by differences in both SDH and PFK activities (SDH, Type II C > A > B; PFK, Type II B > A ≊ C). It is concluded that contractile and metabolic characteristics of human skeletal fibres are very similar to many other species. One difference, however, appears to be that no Type II fibres have an oxidative potential higher than Type I fibres.