High-molecular-weight polypeptide substrates for phospholysine phosphatases.
High-molecular-weight polypeptide substrates for phospholysine phosphatases.
复制标题
磷酸赖氨酸磷酸酶的高分子量多肽底物。
DOI：
10.1006/abio.1994.1447
复制
发表时间：
1994
影响因子：
2.9
通讯作者：
Kennelly,PJ
中科院分区：
文献类型：
--
作者：
Wong,C;Wu,W;Obenshain,SA;Diah,SK;Faiola,B;Kennelly,PJ
负责蛋白质中N-磷酰基（如磷脂酶）去磷酸化的磷酸酶的检测和表征在很大程度上由于缺乏用于测定这些酶的合适底物而受到挫折。在这里，我们描述了制备含磷脂的氨基酸均聚物和杂聚物的化学手段，以及它们在一个简单的，非放射性测定磷脂磷酸酶活性的用途。
The detection and characterization of the phosphatases responsible for the dephosphorylation of N-phosphoryl groups, such as phospholysine, in proteins has been frustrated in large measure by a lack of suitable substrates for the assay of these enzymes. Herein we describe the preparation of phospholysine-containing amino acid homo- and heteropolymers by chemical means, and their use in a simple, nonradioactive assay for phospholysine phosphatase activity.