siRNA off-target effects in genome-wide screens identify signaling pathway members
siRNA off-target effects in genome-wide screens identify signaling pathway members
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DOI：
10.1038/srep00428
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发表时间：
2012-05-29
影响因子：
4.6
通讯作者：
Ferrer, Marc
中科院分区：
文献类型：
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作者：
Buehler, Eugen;Khan, Aly A.;Ferrer, Marc
我们介绍了一种完全基于脱靶效应分析小干扰 RNA (siRNA) 遗传筛选的方法。通过对 Wnt 通路成员进行筛选，我们证明该方法可以识别已知的通路成分，其中一些成分不存在于筛选文库中。该技术可追溯应用于 siRNA 筛选结果，以确认阳性结果并识别使用传统方法进行目标基因选择时遗漏的基因。
We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection.