The source of fatty acids incorporated into proliferating lymphoid cells in immune-stimulated lymph nodes
The source of fatty acids incorporated into proliferating lymphoid cells in immune-stimulated lymph nodes
复制标题
DOI：
10.1079/bjn2002784
复制
发表时间：
2003-03-01
影响因子：
3.6
通讯作者：
Mattacks, CA
中科院分区：
文献类型：
--
作者：
Pond, CM;Mattacks, CA
为了探索免疫刺激淋巴结中增殖的淋巴细胞从邻近脂肪组织局部获取营养的假设，成年豚鼠用标准饲料或补充有 100 g 羊脂、葵花籽油或鱼油/kg 的饲料喂养 6 周。所有豚鼠在最后5天吃标准食物，期间通过反复局部注射脂多糖刺激一侧腘淋巴结肿胀。通过 GC 测定了腘窝和几个肠系膜淋巴结中的磷脂的脂肪酸组成，以及根据其与淋巴结的解剖关系定义的 11 个脂肪组织样品中的三酰甘油的脂肪酸组成。从受刺激的腘淋巴结中提取的磷脂中的脂肪酸比例与周围脂肪细胞中的三酰甘油的比例相关性最好，与淋巴组织相关的其他部位的脂肪细胞的相关性较差，但与无淋巴结的脂肪细胞的相关性较差。在局部免疫刺激下，节周脂肪组织中三酰甘油的组成发生变化。我们得出的结论是，活化淋巴结中增殖的淋巴细胞主要从邻近节周脂肪组织中的三酰甘油获取脂肪酸。免疫刺激会促使含有节点的脂肪细胞三酰甘油的脂肪酸组成发生变化，这些节点使脂肪组织能够提供免疫反应。这种局部相互作用表明，专门的脂肪细胞可以充当全身营养和细胞营养之间的界面，并且可以解释为什么哺乳动物脂肪组织被分为几个大的和许多小的储存库。
To explore the hypothesis that proliferating lymphoid cells in immune-stimulated lymph nodes obtain nutrients locally from adjacent adipose tissue, adult guinea pigs were fed for 6 weeks on standard chow or on chow supplemented with 100 g suet, sunflower oil or fish oil/kg. All the guinea pigs ate standard chow for the last 5 d, during which swelling of one popliteal lymph node was stimulated by repeated local injection of lipopolysaccharide. The fatty acid compositions of phospholipids in both popliteal and in several mesenteric lymph nodes, and of triacylglycerols in eleven samples of adipose tissue defined by their anatomical relations to lymph nodes, were determined by GC. The proportions of fatty acids in the phospholipids extracted from the stimulated popliteal node correlated best with those of triacylglycerols in the surrounding adipocytes, less strongly with those of adipocytes elsewhere in depots associated with lymphoid tissue, but not with those of nodeless depots. The composition of triacylglycerols in the perinodal adipose tissue changed under local immune stimulation. We conclude that proliferating lymphoid cells in activated lymph nodes obtain fatty acids mainly from the triacylglycerols in adjacent perinodal adipose tissue. Immune stimulation prompts changes in the fatty acid composition of the triacylglycerols of adipocytes in node-containing depots that equip the adipose tissue for provisioning immune responses. Such local interactions show that specialised adipocytes can act as an interface between whole-body and cellular nutrition, and may explain why mammalian adipose tissue is partitioned into a few large and many small depots.