Clustering of mutations inactivating a Chi recombinational hotspot.
Clustering of mutations inactivating a Chi recombinational hotspot.
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突变的聚集使 Chi 重组热点失活。
DOI：
10.1016/0022-2836(81)90388-0
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发表时间：
1981
影响因子：
5.6
通讯作者：
Smith,GR
中科院分区：
文献类型：
--
作者：
Schultz,DW;Swindle,J;Smith,GR
Chi位点促进λ噬菌体中的Rec-mediated重组。在这些位点之一χ+C中获得了9个独立的亚硝酸诱导的突变。其中8个突变完全失活了Chi位点，而一个突变留下了部分活性。核苷酸序列分析表明，突变位于四个不同的位点，距离产生活性Chi基因座的χ+C突变位点1至4个碱基对。该间隔在χ+C基因座和另一个测序的Chi基因座χ+B共有的同源区域内。这些结果支持了Chi是一个独特的核苷酸序列的观点，并表明了Chi序列的范围。
Chi sites promote Rec-mediated recombination in bacteriophage λ. Nine independent, nitrous acid-induced mutations were obtained within one of these sites, χ+C. Eight of the mutations completely inactivated the Chi site, while one mutation left partial activity. Nucleotide sequence analysis showed that the mutations were located at four different sites one to four base-pairs from the site of the χ+C mutation that created the active Chi locus. This interval is within a region of homology common to the χ+C locus and another sequenced Chi locus, χ+B. These results support the view that Chi is a unique nucleotide sequence and suggest the extent of the Chi sequence.