pH-based cation exchange chromatography in the capture and elution of monoclonal antibodies
pH-based cation exchange chromatography in the capture and elution of monoclonal antibodies
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DOI：
10.1002/jssc.201100720
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Snyder, Mark A.
中科院分区：
文献类型：
--
作者：
Ng, Paul K.;Snyder, Mark A.
阳离子交换色谱法通过控制盐浓度或流动相ph来分离吸附的蛋白质。本研究探讨了基于ph的单克隆抗体（mab）结合和洗脱方法。对5个等电点在6 ~ 9之间的不同无性系进行了评价。我们使用一种具有高结合能力的新型色谱树脂（Nuvia (TM) S）进行了研究。采用Nuvia S作为捕获步骤的三柱工艺也演示了从组织培养液中纯化单克隆抗体的方法。在50个循环的研究中，证实了Nuvia S的色谱性能。
Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pH-based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evaluated. We performed our studies using a new chromatography resin (Nuvia (TM) S), which has high binding capacity. A three-column process incorporating Nuvia S as a capture step was also demonstrated for the purification of MAb from tissue culture fluid. Chromatography performance of Nuvia S was demonstrated in a 50-cycle study.