Changes in gene transcription and protein expression involved in the response of Agrobacterium sp. ATCC 31749 to nitrogen availability during curdlan production
Changes in gene transcription and protein expression involved in the response of Agrobacterium sp. ATCC 31749 to nitrogen availability during curdlan production
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农杆菌响应中涉及的基因转录和蛋白质表达的变化。
DOI：
10.1134/s0003683811050188
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发表时间：
2011-09
影响因子：
5
通讯作者：
Jian-Rong Wu
中科院分区：
文献类型：
--
作者：
Chi-Chung Lin;Zhi-Yong Zheng;Li-Jun Yu;Xiao-Bei Zhan;Jian-Rong Wu
首先分析了参与氮代谢和可多聚糖生物合成的基因转录和总蛋白表达的变化，以确定农杆菌对该菌的响应。Atcc 31749对柯德兰发酵过程中氮源利用率的影响。在氮素限制条件下，所有氮代谢和调控基因的转录均显著增加。碳(ExoC)和氮(ntrB、ntRC和nifR)代谢基因对氮素限制表现出不同的转录反应。其相对表达水平分别提高了14倍、9倍、7倍和7倍。双向凝胶电泳法(2-DE)显示，在氮饥饿条件下，14个蛋白质的表达显著上调，6个蛋白质的表达显著下调。此外，还鉴定了4种蛋白(GroEL、ABC转运蛋白、Atu1730和烯酰基载体蛋白还原酶)，它们的表达水平发生了显著变化。结果表明，L(音译)是该菌的主要病原。有效地调节其碳通量和氮同化，以获得更好的生存。
The changes in transcription of genes involved in nitrogen metabolism and curdlan biosynthesis, and total protein expression were firstly analyzed to define the responses of Agrobacterium sp. ATCC 31749 to nitrogen source availability during curdlan fermentation. The transcription of all nitrogen metabolism and regulation genes increased significantly under nitrogen limitation. The genes of carbon (exoC) and nitrogen (ntrB, ntrC, and nifR) metabolism showed distinctive transcriptional responses to nitrogen limitation. Their relative expression level was increased by 14, 9, 7 and 7-fold, respectively. Two-dimentional electrophoresis (2-DE) revealed that the expression of 14 proteins were elevated and 6 proteins were down-regulated significantly under nitrogen starvation. Furthermore, 4 proteins (GroEL, ABC transporter, Atu1730 and enoylacyl carrier protein reductase) in which the expression level changed significantly were identified. The results showed that L sp. regulates its carbon flux and nitrogen assimilation effectively for better survival.
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影响因子：
8.4
作者：
Brautaset, Trygve;Petersen, Steffen B.;Valla, Svein
通讯作者：
Valla, Svein
影响因子：
3.2
作者：
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通讯作者：
L. Eberl;A. Ammendola;M. Rothballer;M. Givskov;C. Sternberg;M. Kilstrup;K. Schleifer;S. Molin
影响因子：
6.4
作者：
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通讯作者：
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影响因子：
2.9
作者：
Candiano, G;Bruschi, M;Righetti, PG
通讯作者：
Righetti, PG
影响因子：
3.2
作者：
Z. Zheng;J. W. Lee;X. Zhan;Zhongping Shi;Lei Wang;Li Zhu;Jianrong Wu;C. Lin
通讯作者：
Z. Zheng;J. W. Lee;X. Zhan;Zhongping Shi;Lei Wang;Li Zhu;Jianrong Wu;C. Lin