Transient DNA damage induced by high-frequency electromagnetic fields (GSM 1.8 GHz) in the human trophoblast HTR-8/SVneo cell line evaluated with the alkaline comet assay
Transient DNA damage induced by high-frequency electromagnetic fields (GSM 1.8 GHz) in the human trophoblast HTR-8/SVneo cell line evaluated with the alkaline comet assay
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DOI：
10.1016/j.mrfmmm.2009.10.004
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发表时间：
2010-01-05
影响因子：
2.3
通讯作者：
Fabbri, Elena
中科院分区：
文献类型：
--
作者：
Franzellitti, Silvia;Valbonesi, Paola;Fabbri, Elena
关于高频电磁场（HF-EMF）最有争议的问题之一是它们影响DNA完整性的假定能力。由于高频电动势在通信技术（包括移动的电话）中的使用越来越多，这尤其令人担忧。虽然流行病学研究报告对人类健康没有不利影响，但高频电磁场对细胞生理学可能产生的干扰仍然存在争议。此外，问题仍然是细胞是否能够补偿其潜在的影响。我们以前曾报道过，1小时暴露于调幅1.8 GHz正弦波（GSM-217 Hz，SAR = 2 W/kg）主要用于移动的电话没有引起人类滋养层HTR-8/SVneo细胞的初级DNA损伤水平增加。然而，滋养层细胞反应暴露于GSM信号的不同类型和持续时间的进一步调查被认为是感兴趣的。在目前的工作中，HTR-8/SVneo细胞暴露于1.8 GHz的连续波（CW）和不同的GSM信号，即GSM-217 Hz和GSM-Talk（间歇暴露：5分钟场，10分钟场关闭）4，16或24小时。碱性彗星试验用于评估HF-EMF暴露样品中由于未完成的修复过程而导致的初级DNA损伤和/或链断裂。调幅信号GSM-217 Hz和GSM-Talk在暴露16和24 h后诱导滋养层细胞彗星参数显著增加，而未调制的CW无效。然而，改变迅速恢复和HF-EMF暴露的细胞的DNA完整性是相似的假暴露的细胞在2小时内恢复在没有照射。我们的数据表明，高频电动势的载波频率和调制方案的GSM信号的典型可能会影响DNA的完整性。(C)2009 Elsevier B. V.保留所有权利。
One of the most controversial issue regarding high-frequency electromagnetic fields (HF-EMF) is their putative capacity to affect DNA integrity. This is of particular concern due to the increasing use of HF-EMF in communication technologies, including mobile phones. Although epidemiological studies report no detrimental effects on human health, the possible disturbance generated by HF-EMF on cell physiology remains controversial. In addition, the question remains as to whether cells are able to compensate their potential effects. We have previously reported that a 1-h exposure to amplitude-modulated 1.8 GHz sinusoidal waves (GSM-217 Hz, SAR = 2 W/kg) largely used in mobile telephony did not cause increased levels of primary DNA damage in human trophoblast HTR-8/SVneo cells. Nevertheless, further investigations on trophoblast cell responses after exposure to GSM signals of different types and durations were considered of interest. In the present work, HTR-8/SVneo cells were exposed for 4, 16 or 24 h to 1.8 GHz continuous wave (CW) and different GSM signals, namely GSM-217 Hz and GSM-Talk (intermittent exposure: 5 min field on, 10 min field off). The alkaline comet assay was used to evaluate primary DNA damages and/or strand breaks due to uncompleted repair processes in HF-EMF exposed samples. The amplitude-modulated signals GSM-217 Hz and GSM-Talk induced a significant increase in comet parameters in trophoblast cells after 16 and 24 h of exposure, while the un-modulated CW was ineffective. However, alterations were rapidly recovered and the DNA integrity of HF-EMF exposed cells was similar to that of sham-exposed cells within 2 h of recovery in the absence irradiation. Our data suggest that HF-EMF with a carrier frequency and modulation scheme typical of the GSM signal may affect the DNA integrity. (C) 2009 Elsevier B.V. All rights reserved.