Microsomal Flavin‐Containing Monooxygenase Activity in Rat Corpus Striatum
Microsomal Flavin‐Containing Monooxygenase Activity in Rat Corpus Striatum
复制标题
大鼠纹状体微粒体含黄素单加氧酶活性
DOI：
--
复制
发表时间：
1984
影响因子：
4.7
通讯作者：
Stephen G. Gillespie
中科院分区：
文献类型：
--
作者：
M. Duffel;Stephen G. Gillespie
翻译后摘要：从大鼠纹状体分离的微粒体组分催化氧化硫代苯甲酰胺的亚砜。硫代苯甲酰胺磺基氧化的速率为6.9 ± 4.8 nmol（min）−1（mg微粒体蛋白）−1。该反应被微粒体含黄素单加氧酶的过量含硫和含氮底物抑制。这些硫代苯甲酰胺磺基氧化的抑制剂包括甲巯咪唑、半胱胺和三甲胺。在60± 1 min下处理微粒体制剂也会破坏酶活性。在平行实验中，大鼠肝微粒体表现出相似的抑制特征。数据表明，纹状体中存在一种微粒体单加氧酶，具有肝微粒体含黄素单加氧酶的催化特性。
Abstract: Microsomal fractions isolated from rat corpus striatum catalyze the oxidation of thiobenzamide to the sulfoxide. The rate of thiobenzamide sulfoxidation is 6.9 ± 4.8 nmol (min)−1 (mg microsomal protein)−1. The reaction is inhibited by an excess of sulfur‐and nitrogen‐containing substrates for the microsomal flavin‐containing monooxygenase. These inhibitors of thiobenzamide sulfoxidation include methimazole, cysteamine, and trimethylamine. Enzyme activity is also destroyed by treatment of the microsomal preparation at 60± for 1 min. In parallel experiments, rat liver microsomes exhibit similar inhibition characteristics. The data indicate the presence in corpus striatum of a microsomal monooxygenase with catalytic properties of the hepatic microsomal flavin‐containing monooxygenase.