Inference of photosynthetic capacity parameters from chlorophyll a fluorescence is affected by redox state of PSII reaction centers
Inference of photosynthetic capacity parameters from chlorophyll a fluorescence is affected by redox state of PSII reaction centers
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DOI：
10.1111/pce.14271
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发表时间：
2022-02-06
影响因子：
7.3
通讯作者：
Sun, Ying
中科院分区：
文献类型：
--
作者：
Han, Jimei;Gu, Lianhong;Sun, Ying
太阳能诱导的叶绿素荧光（SIF）已被用于推断光合能力参数（例如，最大羧化速率V-cmax和最大电子传递速率J（max））。然而，这种方法在动态环境下的确切机制和实际效用仍然不清楚。我们使用的光和碳反应之间的平衡，推导出有关叶绿素a荧光（ChlF）发射和光合能力参数的理论方程，并制定了可检验的假设，从PSII（SIFPSII）和V-cmax和J（max）之间的动态关系的真正的总ChlF发射。我们采用了并行测量的气体交换和叶绿素参数为15个物种从6个生物群来测试制定的假设跨物种，温度和限制状态的羧化。我们的研究结果表明，SIFPSII单独是无法通知的V-cmax和J（最大值）跨物种的变化，即使当SIFPSII是在相同的环境条件下确定。相反，SIFPSII和开放PSII反应的分数q（L），这表明PSII的氧化还原状态的产品，是一个强有力的预测V-cmax和J（max），虽然他们的精确关系有所不同的环境条件。我们的研究结果表明，PSII的氧化还原状态强烈影响SIFPSII和V-cmax和J（max）之间的关系。
Solar-induced chlorophyll fluorescence (SIF) has been used to infer photosynthetic capacity parameters (e.g., the maximum carboxylation rate V-cmax, and the maximum electron transport rate J(max)). However, the precise mechanism and practical utility of such approach under dynamic environments remain unclear. We used the balance between the light and carbon reactions to derive theoretical equations relating chlorophyll a fluorescence (ChlF) emission and photosynthetic capacity parameters, and formulated testable hypotheses regarding the dynamic relationships between the true total ChlF emitted from PSII (SIFPSII) and V-cmax and J(max). We employed concurrent measurements of gas exchanges and ChlF parameters for 15 species from six biomes to test the formulated hypotheses across species, temperatures, and limitation state of carboxylation. Our results revealed that SIFPSII alone is incapable of informing the variations in V-cmax and J(max) across species, even when SIFPSII is determined under the same environmental conditions. In contrast, the product of SIFPSII and the fraction of open PSII reactions q(L), which indicates the redox state of PSII, is a strong predictor of both V-cmax and J(max), although their precise relationships vary somewhat with environmental conditions. Our findings suggest the redox state of PSII strongly influences the relationship between SIFPSII and V-cmax and J(max).