Cell-cycle-dependent polar morphogenesis in Caulobacter crescentus: roles of phospholipid, DNA, and protein syntheses.
Cell-cycle-dependent polar morphogenesis in Caulobacter crescentus: roles of phospholipid, DNA, and protein syntheses.
复制标题
新月柄杆菌中细胞周期依赖性极性形态发生：磷脂、DNA 和蛋白质合成的作用。
DOI：
10.1128/jb.171.9.4814-4820.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
Bender,RA
中科院分区：
文献类型：
--
作者：
O'Neill,EA;Bender,RA
在新月柄杆菌中的群集细胞分化期间，群集极处的形态发生的特征在于鞭毛的丧失、噬菌体受体活性（PRA）（细胞吸附噬菌体phi CbK的能力）的丧失，以及最后在先前由鞭毛和PRA占据的位点处开始柄生长。我们在这里表明，这些事件中的每一个都是细胞周期依赖性事件，需要连续的蛋白质合成来执行，但通常发生在没有DNA合成或磷脂合成的情况下。在柄细胞分化过程中，鞭毛和PRA重新出现，柄显著伸长。我们在这里表明，这些事件也依赖于细胞周期，不仅需要从头蛋白质合成，但也DNA和磷脂合成。当使用收集后160分钟分裂的同步细胞时，PRA再现发生在110分钟。这种PRA再现依赖于在70分钟发生的需要磷脂合成的事件，在95分钟发生的需要DNA合成的事件，和在108分钟发生的需要蛋白质合成的事件。在没有净磷脂合成的情况下，茎伸长似乎或多或少正常，但茎最终变得脆弱，到240分钟时，大部分茎已经折断，只留下附着在细胞体上的残根。
During swarmer cell differentiation in Caulobacter crescentus, morphogenesis at the swarmer pole is characterized by the loss of the flagellum, by the loss of phage receptor activity (PRA) (the ability of the cell to adsorb phage phi CbK), and finally by the initiation of stalk outgrowth at the site formerly occupied by the flagellum and the PRA. We show here that each of these events is a cell cycle-dependent event requiring continuous protein synthesis for its execution but occurring normally in the absence of DNA synthesis or phospholipid synthesis. During stalked-cell differentiation, the flagellum and PRA reappear and the stalk elongates considerably. We show here that these events are also cell cycle dependent, requiring not only de novo protein synthesis but also DNA and phospholipid syntheses. When synchronous cells dividing 160 min after collection were used, PRA reappearance occurred at 110 min. This PRA reappearance was dependent on a phospholipid synthesis-requiring event occurring at 70 min, a DNA synthesis-requiring event occurring at 95 min, and a protein synthesis-requiring event occurring at 108 min. In the absence of net phospholipid synthesis, stalk elongation appeared more or less normal, but the stalks eventually became fragile, and by 240 min, most of the stalks had broken off, leaving only stubs attached to the cell body.