Integrating Genetics and Metapopulation Viability Analysis to Inform Translocation Efforts for the Last Northern Leopard Frog Population in Washington State, USA
Integrating Genetics and Metapopulation Viability Analysis to Inform Translocation Efforts for the Last Northern Leopard Frog Population in Washington State, USA
复制标题
整合遗传学和种群活力分析，为美国华盛顿州最后的北方豹蛙种群的易位工作提供信息
DOI：
10.1670/19-097
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发表时间：
2020
影响因子：
0.8
通讯作者：
C. Goldberg
中科院分区：
文献类型：
--
作者：
T. Seaborn;C. Goldberg
摘要。种群遗传分析和种群生存力分析是评估受保护种群状况的有力工具。然而，这两种方法很少结合在一起。为了更好地了解美国华盛顿州最后一个已知的北豹蛙（Lithobates pipiens）种群的状况，并探讨易位的可能性，我们利用7个微卫星位点分析了2004年的96个遗传样本和2014年的294个遗传样本，并将种群遗传数据与种群生存力模型相结合。遗传多样性的划分表明该地区存在三个亚种群，与华盛顿鱼类和野生动物部门在流域内的亚单位名称一致。在这两年中，所有亚群的杂合度和每个位点的平均等位基因数都很低（分别为0.20-0.36和1.86-2.50）。基于G″ST（一种基因分化的测量方法）和BayesAss的迁移估计，三个亚群中有两个的基因流量点估计值从2004年到2014年下降。通过时间估计估计整个地区的有效种群规模很小，不确定性很大（98.4个个体，95%置信区间：15.6个无穷个体）。种群生存力模型利用成虫数量和迁移率的遗传估计表明，成虫可能是成功直接迁移的最佳生活史阶段；然而，由于只能常规地收集到变质虫，因此对先头开始的易位进行了建模，并且可能需要积极的管理策略来建立新的种群。
Abstract. Population genetic and population viability analyses are powerful instruments that can be used to evaluate the status o populations of conservation concern. However, these two methods are rarely integrated. To better understand the status of the last know Northern Leopard Frog (Lithobates pipiens) population in Washington state, USA, and investigate the potential for translocation efforts we analyzed 96 genetic samples from 2004 and 294 genetic samples from 2014 by using seven microsatellite loci and integrate population genetic data with population viability models. Partitioning of genetic diversity indicated the presence of thre subpopulations in the area, aligning with Washington Department of Fish and Wildlife subunit designation within the watershed. I both years, heterozygosity and average number of alleles per locus were low for all subpopulations (0.20–0.36 and 1.86–2.50, respectively) Point estimates of gene flow out of two of the three subpopulations decreased from 2004 to 2014 based on G″ST (a measure of geneti differentiation) and migration estimates from BayesAss. Summed effective population size for the entire area by using tempora estimates was estimated to be small, with large amounts of uncertainty (sum of 98.4 individuals, 95% confidence interval: 15.6–infinit individuals). Population viability models using genetic estimates of number of adults and migration rates suggest that adults may be th optimal life history stage for successful direct translocation; however, because only metamorphs can be routinely collected, translocatio with head-starting was modeled and active management strategies may be necessary to establish new populations.
影响因子：
3.3
作者：
J. K. Pritchard;Matthew Stephens;Peter Donnelly
通讯作者：
J. K. Pritchard;Matthew Stephens;Peter Donnelly
影响因子：
3.3
作者：
G. Wilson;B. Rannala
通讯作者：
G. Wilson;B. Rannala
FORMATOMATIC：用于在群体遗传分析的通用格式之间转换二倍体等位基因数据的程序。
DOI：
10.1111/j.1471-8286.2007.01784.x
发表时间：
2007
期刊：
Molecular ecology notes
影响因子：
--
作者：
Manoukis,NicholasC
通讯作者：
Manoukis,NicholasC