Effect of Mastication on Functional Recoveries after Permanent Middle Cerebral Artery Occlusion in Rats
Effect of Mastication on Functional Recoveries after Permanent Middle Cerebral Artery Occlusion in Rats
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DOI：
10.1016/j.jstrokecerebrovasdis.2009.07.011
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发表时间：
2010-09-01
影响因子：
2.5
通讯作者：
Hirai, Toshihiro
中科院分区：
文献类型：
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作者：
Kawanishi, Katsuya;Koshino, Hisashi;Hirai, Toshihiro
目的：我们评估了对于永久性大脑中动脉闭塞（MCAO）后缺血性脑损伤大鼠的功能恢复，固体饲料是否比液体饲料更有效。方法：对36只雄性Wistar/ST大鼠进行MCAO或假手术。 MCAO或假手术后，所有大鼠均接受液体饲料14天。然后将所有大鼠分为3组：固体喂养组、液体喂养组和假手术组。通过肢体放置试验和莫里斯水迷宫（MWM）任务评估咀嚼对大鼠永久性MCAO后功能恢复的影响。结果：手术后，两个 MCAO 组的肢体放置测试分数相同。在MWM任务的习得试验中，在所有天中液体喂养组和假手术组之间以及在试验的第3天和第4天固体喂养组和假手术组之间观察到逃避潜伏期的显着差异。在探索试验中，液体喂养组和假手术组之间观察到花费的时间存在统计学上的显着差异。在采集试验的第 5 天，3 组在 MWM 任务中在池外围花费的时间存在显着差异。结论：本研究表明固体饮食咀嚼对于脑梗塞引起的感觉运动和学习记忆功能障碍的康复有效。
Objectives: We evaluated whether solid feed is more effective for functional recoveries than liquid feed in rats with ischemic brain injury after permanent middle cerebral artery occlusion (MCAO). Methods: A total of 36 male Wistar/ST rats were subjected to MCAO or sham surgery. After MCAO or sham surgery, all rats were provided liquid feed for 14 days. Then, all rats were divided into 3 groups: the solid feeding group, the liquid feeding group, and the solid feeding group of sham. Effect of mastication on functional recoveries after permanent MCAO in rats was evaluated by the limb placement test and Morris water maze (MWM) task. Results: After surgery, limb placement test scores were equal in both MCAO groups. In the acquisition trials of MWM task, statistically significant differences in escape latency were observed between the liquid feeding group and sham groups at all days, and between the solid feeding group and sham groups at days 3 and 4 of the trials. In the probe trial, statistically significant differences in time spent were observed between the liquid feeding group and sham group. On day 5 of acquisition trials, the time spent in the periphery of the pool in MWM task was significantly different among the 3 groups. Conclusion: This study suggested that solid diet mastication could be effective for the rehabilitation of sensorimotor and learning/memory dysfunction induced by cerebral infarction.