Studies of bronchoalveolar lavage cells and fluids in pulmonary sarcoidosis. II. Enhanced capacity of bronchoalveolar lavage fluids from patients with pulmonary sarcoidosis to induce cell movement in vitro.
Studies of bronchoalveolar lavage cells and fluids in pulmonary sarcoidosis. II. Enhanced capacity of bronchoalveolar lavage fluids from patients with pulmonary sarcoidosis to induce cell movement in vitro.
复制标题
肺结节病支气管肺泡灌洗细胞和液体的研究。
DOI：
10.1164/ajrccm/140.5.1450
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Auerbach,R
中科院分区：
文献类型：
--
作者：
Weber,J;Meyer,KC;Banda,P;Calhoun,WJ;Auerbach,R
在体外增加内皮细胞运动的能力是大多数(如果不是全部)血管生成诱导因子的共同属性。由于肺结节病患者的支气管肺泡灌洗(BAL)细胞诱导新生血管的能力增强，因此评估了这些患者的BAL液对来自不同组织来源的人和小鼠内皮细胞和成纤维细胞的影响。最近开发的计算机辅助图像分析系统被用来在微孔筛选试验中确定细胞迁移的程度和模式。数据来自10名肺结节病患者和5名正常志愿者的BAL液。活动期结节病患者的BAL上清液显示出诱导内皮细胞和成纤维细胞趋化运动的能力增强(2倍至S倍)，这是通过增加迁移面积和极化细胞运动来衡量的。来自不同器官来源的细胞的运动能力存在明显的异质性，但内皮细胞和成纤维细胞的运动能力都有所增强。相比之下，正常人和结节病患者的BAL液对肌肉细胞和尿路细胞的作用相似，而对正常人或非肉芽肿性肺部疾病患者的BAL液有反应的Perl细胞则被肺结节病患者的BAL液抑制。单个培养上清液在体外诱导内皮细胞运动通常与这些患者的BAL细胞在体内诱导血管生成的能力有关。我们认为从肺结节病患者的SAL液中发现的细胞迁移诱导因子可能不仅在肺内肉芽肿形成的细胞募集中发挥重要作用，而且更广泛地在全身结节病的微血管病变中发挥重要作用。
The ability to increase the motility of endothelial cells In vitro Is a property common to most if not all angiogenesis-Inducing factors. Because bronchoalveolar lavage (BAL) cells from patients with pulmonary sarcoidosis havean enhanced capacity to Induce neovascularlzatlon, the BAL fluids from these patients were assessed for their effeCt on human and murine endothelial cells and fibroblasts obtained from a variety of tissue sources. A recently developed computerassisted Image analysis system was used to determine the extent and pattern of cell migration In a mlcrowell screening assay. Data were obtained for BAL fluids from 10 patients with pulmonary sarcoidosis and from five normal volunteers. BAL supernatants from patients with active sarcoidosis showed an enhanced (2-to S-fold) capacity to Induce chemokinesis of both endothelial cells and fibroblasts, as measured by Increased area of migration and polarized cell movement. There was a marked heterogeneity In the motility of cells from different organ origins, but enhanced cell movement was observed with both endothelial cells and fibroblasts. In contrast, BAL fluids from normal and sarcoid patients were similar In their effect on muscle cells and urothellal cells, whereas perlcytes, which responded to BAL fluids from normal subjects or patients with nongranulomatous pulmonary disease, were inhibited by BAL fluids from patients with pulmonary sarcoidosis. The induction of endothelial cell movement in vitro induced by individual supernatants generally correlated with the capacity of BAL cells from these patients to Induce angiogenesis in vivo. Wesuggest that the cell migration-inducing factors found In SAL fluids obtained from patients with pulmonary sarcoidosis may playa significant role not only In recruiting cells for granuloma formation in the lung but more generally in the mlcroangiopathies of systemic sarcoidosis.