A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATA1 signaling pathway
A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATA1 signaling pathway
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DOI：
10.1073/pnas.94.19.10467
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发表时间：
1997-09-16
影响因子：
11.1
通讯作者：
Meyerowitz, EM
中科院分区：
文献类型：
--
作者：
Williams, RW;Wilson, JM;Meyerowitz, EM
植物的持续生长和发育是由分生组织完成的，分生组织是一组未分化的细胞，它们作为干细胞和初始器官存在。虽然双子叶植物的顶端和花分生组织的结构已经被很好地描述，但对这些结构中控制细胞增殖和分化的潜在分子机制知之甚少。我们之前已经证明，拟南芥中的CLAVATA1 （CLV1）基因编码一种受体激酶样蛋白，控制顶端和花分生组织的大小。在这里，我们发现KAPP，一种编码激酶相关蛋白磷酸酶的基因，与CLV1一起在顶端和年轻的花分生组织中表达。此外，clv1具有激酶活性：它磷酸化自身和KAPP，最后，KAPP结合并使clv1去磷酸化。我们提出了一个模型，其中KAPP作为CLAVATA1信号转导通路的负调节因子。
Continuous growth and development in plants are accomplished by meristems, groups of undifferentiated cells that persist as stem cells and initiate organs, While the structures of the apical and floral meristems in dicotyledonous plants have been well described, little is known about the underlying molecular mechanisms controlling cell proliferation and differentiation in these structures. We have shown previously that the CLAVATA1 (CLV1) gene in Arabidopsis encodes a receptor kinase-like protein that controls the size of the apical and floral meristems, Here, we show that KAPP, a gene encoding a kinase-associated protein phosphatase, is expressed in apical and young floral meristems, along with CLV1. Overexpression of KAPP mimics the clv1 mutant phenotype, Furthermore, CLV1 has kinase activity: it phosphorylates both itself and KAPP, Finally, KAPP binds and dephosphorylates CLV1. We present a model where KAPP functions as a negative regulator of the CLAVATA1 signal transduction pathway.