A secreted phosphoprotein marker for neoplastic transformation of both epithelial and fibroblastic cells.
A secreted phosphoprotein marker for neoplastic transformation of both epithelial and fibroblastic cells.
复制标题
用于上皮细胞和成纤维细胞肿瘤转化的分泌性磷蛋白标记物。
作者：
Senger,DR;Asch,BB;Smith,BD;Perruzzi,CA;Dvorak,HF
各种各样的病毒和自发转化的成纤维细胞分泌一种主要的转化相关磷蛋白，其分子量（MW）取决于来源的物种，约为62，000（62 K）1-13。这种主要的32 P标记蛋白质的细胞外水平显著升高并不简单地与指数生长1相关，而是与转化直接相关。磷蛋白与p60 src、p60 c-srcor猿猴病毒40（SV 40）非病毒T抗原2没有抗原相关性，并且基于其电泳迁移率，它与SV 40非病毒T抗原（第53页）进一步区分开来。在这项研究中，我们比较了各种正常和转化上皮细胞分泌的32 P标记的蛋白质，并发现该标记区分肿瘤与癌前和正常小鼠乳腺上皮，也确定了高度致瘤细胞来源于豚鼠胆管上皮和大鼠肝上皮。由于通常与成纤维细胞转化相关的经典表型特性通常不能用于区分致瘤性和非致瘤性上皮细胞4 -9，这种识别成纤维细胞和上皮来源的致瘤性细胞的磷蛋白可能特别重要。
A wide variety of virally and spontaneously transformed fibroblasts secrete a major transformation-related phosphoprotein with a molecular weight (MW), depending on the species of origin, of about 62,000 (62K)1–13. Markedly elevated extracellular levels of this major32P-labelled protein are not simply linked to exponential growth1but instead are associated directly with transformation. The phosphoprotein is not antigenically related to p60src, p60c-srcor simian virus 40 (SV40) non-viral T antigen2, and it is further distinguishable from SV40 non-viral T antigen (pp 53) on the basis of its electrophoretic mobility. In this study we have compared a variety of normal and transformed epithelial cells for secretion of this32P-labelled protein and have found that this marker distinguishes neoplastic from preneoplastic and normal mouse mammary epithelium and also identifies highly tumorigenic cells derived from guinea pig bile duct epithelium and rat liver epithelium. Because the classical phenotypic properties commonly associated with transformation of fibroblasts cannot be generally used to discriminate tumorigenic from non-tumorigenic epithelial cells4–9, this phosphoprotein, which identifies tumorigenic cells of both fibroblastic and epithelial origin, is likely to be of particular importance.