Cytotoxicity and antibacterial property of titanium alloy coated with silver nanoparticle-containing polyelectrolyte multilayer.
Cytotoxicity and antibacterial property of titanium alloy coated with silver nanoparticle-containing polyelectrolyte multilayer.
复制标题
DOI：
10.1016/j.msec.2013.03.010
复制
发表时间：
2013-07
期刊：
影响因子：
--
通讯作者：
Xinming Zhang;Z. Li;Xubo Yuan;Z. Cui;Huijing Bao;Xue Li;Yunde Liu;Xian-Jin Yang
中科院分区：
文献类型：
--
作者：
Xinming Zhang;Z. Li;Xubo Yuan;Z. Cui;Huijing Bao;Xue Li;Yunde Liu;Xian-Jin Yang
将纳米银(AgNP)引入多巴胺修饰的海藻酸钠/壳聚糖(DAL/CHI)多层聚电解质中，对钛合金进行表面修饰，提高其抗菌性能。扫描电子显微镜显示，AgNP均匀包埋在DAL/CHI多层膜中，尺寸约为50 nm。X-射线光电子能谱分析表明，所合成的纳米粒子为银(368.4)，峰值分别为374 eV和374.4 eV。在没有添加还原剂的情况下，银(0)的形成是由于多巴胺的自聚作用，它可以将银阳离子还原为中性金属。聚电解质多层涂层增强了钛合金的润湿性，显著促进了成纤维细胞的增殖，这可能是由于DAL/CHI具有良好的生物相容性。尽管AgNP掺入后L929细胞活性略有下降，但AgNP-DAL/CHI多层膜对大肠杆菌和金黄色葡萄球菌的生长都有抑制作用。以上结果表明，多巴胺修饰是在聚电解质多层膜中原位诱导AgNP形成的一种简单而有效的方法。此外，含AgNP的多层膜显著提高了钛合金的抗菌活性。在钛种植体表面制备AgNP-DAL/CHI多层膜具有很大的骨科应用潜力。
Silver nanoparticle (AgNP) was incorporated into dopamine-modified alginate/chitosan (DAL/CHI) polyelectrolyte multilayer to modify the surface of titanium alloy and improve its antibacterial property. Scanning electron microscopy showed that AgNP with the size of 50nm embedded in DAL/CHI multilayers homogeneously. X-ray photoelectron spectroscopy analysis indicated that the nanoparticles were silver (0) with peaks at 368.4 and 374.4eV, respectively. The formation of silver (0) without the addition of reductants was due to the self-polymerization of dopamine, which can reduce the silver cation into neutral metal. The polyelectrolyte multilayer coating enhanced the wettability of titanium alloy and promoted the fibroblast proliferation significantly, which could be attributed to the excellent biocompatibility of DAL/CHI. Despite the slight fall of L929 cell activity after AgNP incorporation, AgNP-DAL/CHI multilayer inhibited the growth of both Escherichia coli and Staphylococcus aureus. The above results demonstrate that dopamine decoration is a simple and effective way to induce the in-situ formation of AgNP within polyelectrolyte multilayer. Furthermore, the AgNP-containing multilayer considerably enhances the antibacterial activity of titanium alloy. The fabrication of AgNP-DAL/CHI multilayer on the surface of titanium implant might have great potential in orthopedic use.