STIMULATION OF NEURONAL ACETYLCHOLINE-RECEPTORS INDUCES RAPID GENE-TRANSCRIPTION
STIMULATION OF NEURONAL ACETYLCHOLINE-RECEPTORS INDUCES RAPID GENE-TRANSCRIPTION
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DOI：
10.1126/science.3749894
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发表时间：
1986-10-03
期刊：
影响因子：
56.9
通讯作者：
GREENE, LA
中科院分区：
文献类型：
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作者：
GREENBERG, ME;ZIFF, EB;GREENE, LA
胆碱能激动剂快速和瞬时诱导转录的c-fos原核基因和一个或多个肌动蛋白基因在神经元分化的PC 12细胞。转录激活后几分钟内刺激的烟碱乙酰胆碱受体，并需要通过电压敏感性钙通道的细胞外Ca 2+离子的流入。尼古丁的激活通过与神经生长因子激活不同的途径进行，神经生长因子对这些基因的刺激不依赖于细胞外Ca 2+离子。这些发现表明，神经递质可以快速激活特定的基因转录在非分裂的神经元分化的细胞。他们还建议在神经系统中的c-fos和肌动蛋白表达的神经递质诱导的功能作用。
Cholinergic agonists rapidly and transiently induced transcription of the c-fos protonocogene and one or more actin genes in neuronally differentiated PC12 cells. Transcription was activated within minutes after stimulation of the nicotinic acetylcholine receptor and required an influx of extracellular Ca2+ ions through voltage-sensitive calcium channels. Nicotine activation proceeded by a different pathway from activation by nerve growth factor, whose stimulation of these genes is independent of extracellular Ca2+ ions. These finding suggest that neurotransmitters may rapidly activate specific gene transcription in nondividing neuronally differentiated cells. They also suggest a functional role for neurotransmitter induction of c-fos and actin expression in the nervous system.