Two-photon absorption of bacteriorhodopsin: formation of a red-shifted thermally stable photoproduct F620.
Two-photon absorption of bacteriorhodopsin: formation of a red-shifted thermally stable photoproduct F620.
复制标题
细菌视紫红质的双光子吸收：红移热稳定光产物 F620 的形成。
作者：
T. Fischer;N. Hampp
利用高强度532 nm激光脉冲，诱导细菌视紫红质（BR）的初始B（570）态光化学转化为在BR悬浮液中在约620 nm处和在BR膜中在约610 nm处最大吸收的稳定光产物。这种状态，我们命名为F（620），被进一步光化学转化为一组三个产物，在340 nm到380 nm波长范围内具有最大吸收，其光谱性质与文献中报道的所谓P（360）状态相同。从B（570）到F（620）的光转换最可能是共振双光子吸收诱导的步骤。F（620）和P（360）的形成导致BR膜具有显著的光致永久光学各向异性。在离析物（B（570））和产物（F（620））波长处的光致各向异性和各向异性取向的光谱依赖性是F（620）在直接光化学步骤中由B（570）形成的强烈指示。P（360）产物的化学性质可能是含有BR的retro-retinal，但F（620）态的结构特征仍不清楚。短激光脉冲在BR膜中引起的光致永久各向异性有助于更好地理解与此转变相关的光化学途径，并且鉴于潜在的应用，这是有趣的，因为这一特征是使用BR膜进行永久光学数据存储的分子基础。
By means of high-intensity 532 nm laser pulses, a photochemical conversion of the initial B(570) state of bacteriorhodopsin (BR) to a stable photoproduct absorbing maximally at approximately 620 nm in BR suspensions and at approximately 610 nm in BR films is induced. This state, which we named F(620), is photochemically further converted to a group of three products with maximal absorptions in the wavelength range from 340 nm to 380 nm, which show identical spectral properties to the so-called P(360) state reported in the literature. The photoconversion from B(570) to F(620) is most likely a resonant two-photon absorption induced step. The formation of F(620) and P(360) leads to a distinguished photo-induced permanent optical anisotropy in BR films. The spectral dependence of the photo-induced anisotropy and the anisotropy orientations at the educt (B(570)) and product (F(620)) wavelengths are strong indicators that F(620) is formed in a direct photochemical step from B(570). The chemical nature of the P(360) products probably is that of a retro-retinal containing BR, but the structural characteristics of the F(620) state are still unclear. The photo-induced permanent anisotropy induced by short laser pulses in BR films helps to better understand the photochemical pathways related to this transition, and it is interesting in view of potential applications as this feature is the molecular basis for permanent optical data storage using BR films.
激光诱导的细菌视紫红质的蓝色状态：蛋白质-蛋白质相互作用、蛋白质-脂质相互作用和金属离子的机械和颜色调节作用。
影响因子：
15
作者：
Masthay,MarkB;Sammeth,DavidM;Helvenston,MerrittC;Buckman,CharlesB;Li,Wuyi;Cde-Baca,MichaelJ;Kofron,JohnT
通讯作者：
Kofron,JohnT