Size-dependent toxicity of nano-C60 aggregates: more sensitive indication by apoptosis-related Bax translocation in cultured human cells.
Size-dependent toxicity of nano-C60 aggregates: more sensitive indication by apoptosis-related Bax translocation in cultured human cells.
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DOI：
10.1021/es2039008
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发表时间：
2012-03
影响因子：
11.4
通讯作者：
Maoyong Song;S. Yuan;Junfa Yin;Xiaoli Wang;Zi-hui Meng;Hailin Wang;G. Jiang
中科院分区：
文献类型：
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作者：
Maoyong Song;S. Yuan;Junfa Yin;Xiaoli Wang;Zi-hui Meng;Hailin Wang;G. Jiang
NPs的毒性并不能很好地描述它们的大小。特别是，富勒烯(C(60))的基于尺寸的毒性仍然是一个问题，因为缺乏尺寸可控的C(60)NPs。在本工作中，通过简单的差示离心法制备了六种不同尺寸分布的纳米C(60)聚集体(NC(60))。通过使用这些NC(60)组分，我们证明了DNA聚合酶的大小依赖抑制和缩小尺寸增强的细胞毒性。最重要的是，我们发现尺寸较小的NC(60)纳米粒可能具有更高的毒性效力。在高暴露剂量(4-6 mg/L)下观察到这些大小依赖效应。有趣的是，在20倍的低剂量和非细胞毒性剂量下，这种大小依赖的效应可以通过凋亡相关荧光蛋白融合的Bax易位来指示。考虑到传统的细胞毒性终点分析在低剂量暴露时往往忽略纳米颗粒的毒性，本文的研究结果将有助于评价低剂量NC(60)纳米颗粒的尺寸依赖性细胞毒性和毒性的剂量-反应关系。
The toxicity of NPs is not well characterized in terms of their size. In particular, the size-based toxicity of fullerene (C(60)) remains an issue because of a lack of C(60) NPs with a well-controlled size. In this work, six fractions of the nano-C(60) aggregates (nC(60)) with different size distribution were prepared by a simple differential centrifugation. By using these nC(60) fractions, we demonstrate the size-dependent inhibition of DNA polymerase and reduced-size enhanced cytotoxicity. Above all, we found that nC(60) NPs with smaller size may have higher toxicity potency. These size-dependent effects were observed at the high exposure doses (4-6 mg/L). Interestingly, at 20-times lower and noncytotoxic doses, the size-dependent effect can be indicated by apoptosis-related fluorescent protein fused Bax translocation. Considering the toxicity of NPs is often ignored in the traditional end-point analysis for cytotoxicity when the exposure dose is low, the findings presented here will assist in the evaluation of the size-dependent cytotoxicity and dose-response relationships of toxicity mediated by nC(60) NPs at low doses.