APSR1, a novel gene required for meristem maintenance, is negatively regulated by low phosphate availability
APSR1, a novel gene required for meristem maintenance, is negatively regulated by low phosphate availability
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DOI：
10.1016/j.plantsci.2012.12.015
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发表时间：
2013-05-01
期刊：
影响因子：
5.2
通讯作者：
Herrera-Estrella, Luis
中科院分区：
文献类型：
--
作者：
Gonzalez-Mendoza, Victor;Zurita-Silva, Andres;Herrera-Estrella, Luis
适当的根系生长对于锚定、探测和利用土壤基质至关重要。根系生长对多种环境因素非常敏感，其中水分和养分有效性对根系发育影响较大。在自然和农业环境下，磷有效性是影响植物生长发育的最具限制性的养分之一。根长轴方向的生长从根尖开始，需要细胞增殖、细胞伸长和细胞分化的协调活动。在这里，我们报道了一个新的基因，APSR1（改变的磷酸盐饥饿反应1），参与根分生组织的维持。功能缺失突变体apsr1-1表现为主根长度和根尖分生组织大小减少，表皮细胞分化短，根毛长。在磷限制条件下，APSR1基因在根尖未分化细胞中未表现出典型的进行性减少。有趣的是，APSR1的表达模式与生长素积累的根区重叠。此外，apsr1-1表现出生长素转运体PIN7水平的明显下降。这些数据表明，APSR1是协调细胞过程所必需的，以正确的根生长应对磷酸盐饥饿，可以想象通过直接或间接调节PIN7。我们还提出，基于其核定位和结构，APSR1可能是一组新的转录因子的潜在成员。2013爱思唯尔爱尔兰有限公司版权所有。
Proper root growth is crucial for anchorage, exploration, and exploitation of the soil substrate. Root growth is highly sensitive to a variety of environmental cues, among them water and nutrient availability have a great impact on root development. Phosphorus (P) availability is one of the most limiting nutrients that affect plant growth and development under natural and agricultural environments. Root growth in the direction of the long axis proceeds from the root tip and requires the coordinated activities of cell proliferation, cell elongation and cell differentiation. Here we report a novel gene, APSR1 (Altered Phosphate Starvation Response1), involved in root meristem maintenance. The loss of function mutant apsr1-1 showed a reduction in primary root length and root apical meristem size, short differentiated epidermal cells and long root hairs. Expression of APSR1 gene decreases in response to phosphate starvation and apsr1-1 did not show the typical progressive decrease of undifferentiated cells at root tip when grown under P limiting conditions. Interestingly, APSR1 expression pattern overlaps with root zones of auxin accumulation. Furthermore, apsr1-1 showed a clear decrease in the level of the auxin transporter PIN7. These data suggest that APSR1 is required for the coordination of cell processes necessary for correct root growth in response to phosphate starvation conceivably by direct or indirect modulation of PIN7. We also propose, based on its nuclear localization and structure, that APSR1 may potentially be a member of a novel group of transcription factors. (C) 2013 Elsevier Ireland Ltd. All rights reserved.