The Properties of A Protease Activated by High Pressure from Sporosarcina sp. strain DSK25 Isolated from Deep-sea Sediment
The Properties of A Protease Activated by High Pressure from Sporosarcina sp. strain DSK25 Isolated from Deep-sea Sediment
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发表时间：
1995-09
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通讯作者：
C. Kato;Seiko Suzuki;S. Hata;Takashi Ito;K. Horikoshi
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作者：
C. Kato;Seiko Suzuki;S. Hata;Takashi Ito;K. Horikoshi
1991年，日本海洋科学技术中心（JAMSTEC）成员在日本海沟6500米深度（40'06.8"N 144'11.0"E）进行了深海调查，使用“新海6500”载人潜水器获得了深海泥浆样本。从泥样中分离出一株深海细菌DSK25，并测定了其若干特性。菌株DSK25属于孢子菌属，产生胞外蛋白酶。菌株DSK25的蛋白酶在pH9和40°C左右具有最佳活性，其活性被苯基甲基磺酰氟（PMSF）完全抑制。推测该蛋白酶为碱性丝氨酸蛋白酶。菌株DSK25的蛋白酶在高压下活性增强，而其他商业蛋白酶在高压下无活性增强。这表明这种蛋白酶可能适应了深海中的高压环境。
From a deep-sea investigation in 1991 by members of the Japan Marine Science and Technology Center (JAMSTEC), deep-sea mud samples were obtained from the Japan Trench at 6500 m depth (40'06.8"N 144'11.0"E), using the manned submersible "SHINKAI 6500". The deep-sea bacterium, strain DSK25 was isolated from the mud samples, and several of its properties were determined. Strain DSK25 belongs to the genus Sporosarcina sp., and produced an extracellular protease. The protease from strain DSK25 had optimal activity at around pH9 and 40°C and its activity was inhibited completely by phenylmethylsulfonyl fluoride (PMSF). It is suggested that this protease is an alkaline serine protease. The activity of the protease from strain DSK25 was enhanced by elevated pressure, whereas other commercial proteases showed no pressure enhancement. This suggests that the protease may be adapted to the high pressure environment found in the deep-sea.