Fluorescent blood glucose monitor by hemin-functionalized graphene quantum dots based sensing system
Fluorescent blood glucose monitor by hemin-functionalized graphene quantum dots based sensing system
复制标题
基于血红素功能化石墨烯量子点的传感系统的荧光血糖监测仪
DOI：
10.1016/j.aca.2013.11.059
复制
发表时间：
2014-01-31
影响因子：
6.2
通讯作者：
Wang, Lun
中科院分区：
文献类型：
--
作者：
He, Yuezhen;Wang, Xiaoxun;Wang, Lun
本文基于氯化血红素功能化的石墨烯量子点（GQDs）和葡萄糖氧化酶（GOx）体系，构建了一种高灵敏度、高特异性的血糖荧光生物传感器。通过柠檬酸热解法简单制备的GQD具有较强的荧光性和良好的水溶性。由于氯化血红素与GQD之间的非共价组装，氯化血红素的加入可以使过氧化氢（H2 O2）破坏GQD的钝化表面，导致GQD的显著荧光猝灭。基于这种效应，提出了一种新型的荧光平台用于葡萄糖的传感。在优化条件下，葡萄糖的线性范围为9 ~ 300 μ M，检测限为0.1 μ M。由于GQDs的独特性质，该传感器具有绿色、简单、成本低的优点，并成功地应用于人血清中葡萄糖的测定。此外，所提出的方法提供了一个新的途径，进一步设计的生物传感器的基础上组装的GQD与氯化血红素检测生物分子。(C)2013爱思唯尔有限公司版权所有。
In the present work, a highly sensitive and specific fluorescent biosensor for blood glucose monitoring is developed based on hemin-functionalized graphene quantum dots (GQDs) and glucose oxidase (GOx) system. The GQDs which are simply prepared by pyrolyzing citric acid exhibit strong fluorescence and good water-solubility. Due to the noncovalent assembly between hemin and GQDs, the addition of hemin can make hydrogen peroxide (H2O2) to destroy the passivated surface of GQDs, leading to significant fluorescence quenching of GQDs. Based on this effect, a novel fluorescent platform is proposed for the sensing of glucose. Under the optimized conditions, the linear range of glucose is from 9 to 300 p,M, and the limit of detection is 0.1 mu M. As unique properties of GQDs, the proposed biosensor is green, simple, cost-efficient, and it is successfully applied to the determination of glucose in human serum. In addition, the proposed method provides a new pathway to further design the biosensors based on the assembly of GQDs with hemin for detection of biomolecules. (C) 2013 Elsevier B.V. All rights reserved.