Proteomic characterization of human early pro-angiogenic cells
Proteomic characterization of human early pro-angiogenic cells
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DOI：
10.1016/j.yjmcc.2010.11.022
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发表时间：
2011-02-01
影响因子：
5
通讯作者：
Mayr, Manuel
中科院分区：
文献类型：
--
作者：
Urbich, Carmen;De Souza, Ayesha I.;Mayr, Manuel
早期促血管生成细胞（EPCs）参与新生血管形成、血管生成和再内皮化，而组织蛋白酶L抑制剂可减弱其促血管生成作用。在本研究中，我们已经分析和映射的蛋白质组和分泌组的人EPCs，利用差异凝胶电泳（DICE）和鸟枪蛋白质组学相结合。分析了206个蛋白质点的群体，其中171个在EPCs的细胞蛋白质组中被鉴定。在它们的条件培养基中鉴定了82种蛋白质，包括替代巨噬细胞标志物C-C基序趋化因子18（CCL 18）和血红蛋白清道夫受体CD 163以及血小板因子4（CXCL 4）和血小板碱性蛋白（CXCL 7），其中“血小板α颗粒”根据基因本体论注释返回为顶部类别。除了组织蛋白酶L，组织蛋白酶L抑制剂还减弱了广泛的其他组织蛋白酶和溶酶体蛋白如豆荚蛋白的释放，但刺激S100蛋白家族成员的分泌。本文提供的数据是迄今为止人类EPCs中蛋白质表达和分泌的最全面表征，并突出了半胱氨酸蛋白酶在血小板因子加工中促血管生成潜力的潜在重要性。这篇文章是题为“再访心血管干细胞”的特刊的一部分。(C)2010爱思唯尔有限公司版权所有。
Early pro-angiogenic cells (EPCs) have been shown to be involved in neovascularization, angiogenesis and reendothelialization and cathepsin L inhibition blunted their pro-angiogenic effect. In the present study, we have analysed and mapped the proteome and secretome of human EPCs, utilizing a combination of difference in-gel electrophoresis (DICE) and shotgun proteomics. A population of 206 protein spots were analysed, with 171 being identified in the cellular proteome of EPCs. 82 proteins were identified in their conditioned medium, including the alternative macrophage markers C-C motif chemokine 18 (CCL18) and the hemoglobin scavenger receptor CD163 as well as platelet factor 4 (CXCL4) and platelet basic protein (CXCL7) with "platelet alpha granule" being returned as the top category according to the Gene Ontology Annotation. Apart from cathepsin L, the cathepsin L inhibitor also attenuated the release of a wide range of other cathepsins and lysosomal proteins such as legumain, but stimulated the secretion of members of the S100 protein family. The data presented here are the most comprehensive characterization of protein expression and secretion in human EPCs to date and highlight the potential importance of cysteine proteases in the processing of platelet factors for their pro-angiogenic potential. This article is part of a special issue entitled, "Cardiovascular Stem Cells Revisited". (C) 2010 Elsevier Ltd. All rights reserved.