rhBMP-2 Pre-Treated Human Periodontal Ligament Stem Cell Sheets Regenerate a Mineralized Layer Mimicking Dental Cementum
rhBMP-2 Pre-Treated Human Periodontal Ligament Stem Cell Sheets Regenerate a Mineralized Layer Mimicking Dental Cementum
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DOI：
10.3390/ijms21113767
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发表时间：
2020-06-01
影响因子：
5.6
通讯作者：
Yun, Jeong-Ho
中科院分区：
文献类型：
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作者：
Park, Joo-Young;Park, Chan Ho;Yun, Jeong-Ho
牙周复合体由牙槽骨、牙骨质和牙周韧带（PDL）组成，通过矿化组织和纤维组织的系统协调来支撑人类的牙齿。重要的是，牙骨质是牙根最外层的矿化层，在连接牙根与牙槽骨之间的内韧带方面起着至关重要的作用。当牙周复合体受损时，需要对牙周复合体的各个组成部分进行再生；然而，实现完整的功能再生仍然具有挑战性。在本研究中，我们尝试采用重组人BMP-2 （rhBMP-2）预处理的人PDL干细胞（hPDLSC）片工程技术来控制牙骨质和PDL的再生。用rhBMP-2预处理离体hPDLSCs进行体外成骨分化，并将其移植到代表牙根的微/大孔双相磷酸钙（MBCP）块上。将带hPDLSC片的MBCPs植入免疫受损小鼠皮下，rhBMP-2预处理组的hPDLSC片矿化和胶原韧带沉积高于未预处理组。因此，rhBMP-2-hPDLSC片技术可能是牙周复合体中矿化组织和纤维组织两种不同组织同步再生的有效策略。
The periodontal complex consisting of alveolar bone, cementum, and periodontal ligaments (PDL) supports human teeth through the systematic orchestration of mineralized tissues and fibrous tissues. Importantly, cementum, the outermost mineralized layer of dental roots, plays an essential role by bridging the inner ligaments from the dental root to the alveolar bone. When the periodontal complex is damaged, the regeneration of each component of the periodontal complex is necessary; however, it is still challenging to achieve complete functional regeneration. In this study, we tried to control the regeneration of cementum and PDL by using a human PDL stem cell (hPDLSC) sheet engineering technology with the pretreatment of recombinant human BMP-2 (rhBMP-2). Isolated hPDLSCs obtained from extracted human teeth were pretreated with rhBMP-2 for in vitro osteogenic differentiation and grafted on the micro/macro-porous biphasic calcium phosphate (MBCP) blocks, which represent dental roots. The MBCPs with hPDLSC sheets were implanted in the subcutaneous layer of immune-compromised mice, and rhBMP-2 pretreated hPDLSC sheets showed higher mineralization and collagen ligament deposition than the no-pretreatment group. Therefore, the rhBMP-2-hPDLSC sheet technique could be an effective strategy for the synchronized regeneration of two different tissues: mineralized tissue and fibrous tissues in periodontal complexes.