Molecular cloning and phylogenetic analysis of small GTPase protein Tscdc42 from Trichinella spiralis
Molecular cloning and phylogenetic analysis of small GTPase protein Tscdc42 from Trichinella spiralis
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旋毛虫小GTP酶蛋白Tscdc42的分子克隆及系统发育分析
DOI：
10.1007/s00436-010-1735-z
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发表时间：
2010-01
影响因子：
2
通讯作者：
Qin, Weiwen
中科院分区：
文献类型：
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作者：
Yang, Yurong;Jian, Wei;Qin, Weiwen
从旋毛虫（一种具有重要经济意义的人兽共患寄生线虫）中分离出了一个全长互补脱氧核糖核酸（Tscdc42），它编码一种假定的Rho家族小GTP酶基因cdc42。其不间断的开放阅读框……
A full-length of complementary deoxyribonucleic acid (Tscdc42) encoding a putative Rho-family small GTPase gene cdc42 was isolated from Trichinella spiralis, an economically important parasitic nematode of zoonosis. The uninterrupted open reading frame of TsCDC42 encodes a predicted protein of 147 amino acids and containing a highly conserved domain of CDC42. Comparison with selected sequences from the free-living nematode Caenorhabditis elegans, Drosophila melanogaster, Xenopus laevis, Danio rerio, Mus musculus, and human showed that Tscdc42 is highly conserved. The highest identity of TsCDC42 with CDC42 from Drosophila is 67%, the similarity is up to 73%, the identity of TsCDC42 with the CDC42 homologue of C. elegans is 62%, and the similarity is up to 71%. Phylogenetic analysis of the amino acid sequence data, using the neighbor-joining and maximum parsimony methods, revealed that TsCDC42 is closely related to the molecule inferred from the cdc-42 gene of C. elegans. The transcript of TsCDC42 was analyzed during different stages of the worm.
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DOI：
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发表时间：
2002
期刊：
--
影响因子：
--
作者：
D. Swofford
通讯作者：
D. Swofford
DOI：
10.1161/01.atv.0000168418.10276.f0
发表时间：
2005-07
期刊：
Arteriosclerosis, Thrombosis, and Vascular Biology
影响因子：
--
作者：
Tatsuya Yufu;K. Hirano;Dan Bi;M. Hirano;J. Nishimura;Y. Iwamoto;H. Kanaide
通讯作者：
Tatsuya Yufu;K. Hirano;Dan Bi;M. Hirano;J. Nishimura;Y. Iwamoto;H. Kanaide
影响因子：
16
作者：
Peterson, FC;Penkert, RR;Prehoda, KE
通讯作者：
Prehoda, KE
影响因子：
25
作者：
Schwamborn, JC;Püschel, AW
通讯作者：
Püschel, AW
DOI：
--
发表时间：
2006
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
D. Solecki;E. Govek;M. Hatten
通讯作者：
D. Solecki;E. Govek;M. Hatten