Gastrointestinal handling of glycosyl [13C]ureides
Gastrointestinal handling of glycosyl [13C]ureides
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糖基[13C]酰脲的胃肠道处理
DOI：
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发表时间：
2003
影响因子：
4.7
通讯作者：
L. Weaver
中科院分区：
文献类型：
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作者：
D. Morrison;B. Dodson;T. Preston;L. Weaver
目的：乳糖[13 C]酰脲已被提议作为成人和儿童口盲肠通过时间的非侵入性标记物。本研究调查了胃肠道对乳糖[13 C]酰脲（13 C LU）和葡萄糖[13 C]酰脲（13 C GU）的处理，描述了这些底物的代谢结局，并描述了不同途径的示踪剂排泄程度。四名受试者接受了五项研究，其中他们摄入了试验餐，外加（1）无底物，（2）13 C LU，（3）13 C GU，（4）用未标记的乳糖酰脲预给药后的13 C LU和（5）用葡萄糖酰脲预给药后的13 C LU。受试者在家中接受研究，两次测试之间至少间隔1周，他们都完成了研究。呼吸进行了分析，13 CO2回收和尿液进行了分析，总13 C回收，13 C尿素回收和13 C GU recovery.Results：的轮廓和程度的示踪剂回收在呼吸和尿液中是相似的，当使用13 C GU或13 C LU，这表明这些底物的肠道类似的处理。13 C GU是主要的13 C-富集的物种回收的尿液中，即使当13 C LU消耗。预给药与乳糖酰脲或葡萄糖酰脲的示踪剂的出现率增加，但没有改变过境times.Conclusions：13 C LU水解为13 C GU在小肠与13 C GU出现在尿中的馏分可能是有限的小肠通透性。13 C LU或13 C GU均可用于测量口盲肠通过时间。赞助：英国苏格兰汉密尔顿贝尔学院和首席科学家办公室。
Objectives: Lactose [13C]ureide has been proposed as a noninvasive marker for oro-caecal transit time in adults and children. The present study investigates the handling of lactose [13C]ureide (13C LU) and glucose [13C]ureide (13C GU) by the gastrointestinal tract and describes the metabolic fates of these substrates and describes the extent of tracer excretion by different routes.Study Design and Subjects: Four subjects underwent five studies in which they ingested a test meal plus (1) no substrate, (2) 13C LU, (3) 13C GU, (4) 13C LU after predosing with unlabelled lactose ureide and (5) 13C LU after predosing with glucose ureide. Subjects were studied at home with at least 1 week between tests and they all completed the study. Breath was analysed for 13CO2 recovery and urine was analysed for total 13C recovery, 13C urea recovery and 13C GU recovery.Results: The profiles and extent of tracer recovery in breath and urine were similar when either 13C GU or 13C LU was used, suggesting similar handling of these substrates by the gut. 13C GU was the major 13C-enriched species recovered in the urine even when 13C LU was consumed. Predosing with either lactose ureide or glucose ureide increased the rate of appearance of tracer, but did not alter transit times.Conclusions: 13C LU is hydrolysed to 13C GU in the small intestine with the fraction of 13C GU appearing in the urine probably limited by small intestinal permeability. Either 13C LU or 13C GU can be used to measure oro-caecal transit time.Sponsorship: Bell College, Hamilton and Chief Scientist Office, Scotland, UK.