Characterisation of lipid fraction of marine macroalgae by means of chromatography techniques coupled to mass spectrometry.
Characterisation of lipid fraction of marine macroalgae by means of chromatography techniques coupled to mass spectrometry.
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DOI：
10.1016/j.foodchem.2013.08.130
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发表时间：
2014-02
期刊：
影响因子：
8.8
通讯作者：
Carla Ragonese;L. Tedone;M. Beccaria;Germana Torre;F. Cichello;F. Cacciola;P. Dugo;L. Mondello
中科院分区：
文献类型：
--
作者：
Carla Ragonese;L. Tedone;M. Beccaria;Germana Torre;F. Cichello;F. Cacciola;P. Dugo;L. Mondello
在这项工作中，报告了沿西西里岛东海岸采集的几种海洋大型藻类的脂质部分的特征。对两种绿色海藻（Chloropyceae）、两种红色海藻（Rhodophyceae）和四种棕色海藻（Pheophyceae）的脂肪酸、三酰甘油、色素和磷脂谱进行了评估。采用先进的分析技术来全面表征这些地中海海藻的脂质谱，例如将 GC-MS 与新型质谱数据库相结合，同时使用线性保留指数 (LRI) 来识别脂肪酸谱； LC-MS 用于鉴定三酰甘油 (TAG)、类胡萝卜素和磷脂；对类胡萝卜素和磷脂进行精确质量测定。以总分数的相对百分比报告脂肪酸和三酰甘油的定量数据。
In this work the characterisation of the lipid fraction of several species of marine macro algae gathered along the eastern coast of Sicily is reported. Two species of green marine algae (Chloropyceae), two species of red marine algae (Rhodophyceae) and four species of brown marine algae (Pheophyceae) were evaluated in terms of fatty acids, triacylglycerols, pigments and phospholipids profile. Advanced analytical techniques were employed to fully characterise the lipid profile of these Mediterranean seaweeds, such as GC–MS coupled to a novel mass spectra database supported by the simultaneous use of linear retention index (LRI) for the identification of fatty acid profile; LC–MS was employed for the identification of triacylglycerols (TAGs), carotenoids and phospholipids; the determination of accurate mass was carried out on carotenoids and phospholipids.Quantitative data are reported on fatty acids and triacylglycerols as relative percentage of total fraction.