Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice
Developmental ability of oocytes retrieved from Meishan neonatal ovarian tissue grafted into nude mice
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DOI：
10.1111/asj.13160
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发表时间：
2019-01
期刊：
影响因子：
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通讯作者：
H. Kaneko;K. Kikuchi;N. Men;J. Noguchi
中科院分区：
文献类型：
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作者：
H. Kaneko;K. Kikuchi;N. Men;J. Noguchi
卵巢异种移植使从西方品种的未成熟猪获得具有受精能力的卵母细胞成为可能。在本研究中，我们将这些方法应用于中国本土猪品种梅山猪，并研究了卵母细胞在其新生组织中移植到裸鼠体内后的发育能力。首先，从阴道开放后10、30和60天开始（分别为D10‐、D30‐和D60‐FSH组），将含有新生卵巢组织的小鼠注入促卵泡激素（FSH） （62.5 U/ml） 13天。在D60‐FSH组中，窦卵泡及其卵母细胞的发育得到了最大的促进。下一步，我们检测了从阴道开放后60天开始，以62.5 U/ml或125 U/ml剂量输注FSH （FSH‐62.5或‐125组）13天后，从宿主小鼠体内恢复的卵母细胞的体外成熟能力。从FSH - 125组获得了更多具有成熟能力的卵母细胞。FSH - 125成熟卵母细胞在体外受精，形成雄性和雌性原核，但未达到囊胚期。上述结果表明，梅山新生儿卵巢移植到裸鼠体内后能够产生具有受精能力的卵母细胞。
Abstract Ovarian xenografting makes it possible to obtain oocytes with fertilization ability from immature pigs of Western breeds. In this study, we applied these methods to the Meishan, an indigenous Chinese pig breed, and investigated the developmental competence of oocytes grown in their neonatal tissue after grafting into nude mice. First, mice harboring neonatal ovarian tissue were infused with follicle stimulating hormone (FSH) (62.5 U/ml) for 13 days starting at 10, 30, and 60 days after vaginal opening (D10‐, D30‐, and D60‐FSH groups, respectively). Development of antral follicles and their oocytes was most enhanced in the D60‐FSH group. For the next step, we examined the in vitro maturation ability of the oocytes recovered from host mice after infusion with FSH at a dose of 62.5 U/ml or 125 U/ml (FSH‐62.5 or ‐125 group) for 13 days starting at 60 days after vaginal opening. Many more oocytes with maturation ability were obtained from the FSH‐125 group. The FSH‐125 mature oocytes were fertilized in vitro, as shown by formation of male and female pronuclei, but did not reach the blastocyst stage. These results indicate that Meishan neonatal ovaries are able to produce oocytes with fertilization ability after being grafted into nude mice.