SEX-RELATED AND STRAIN-RELATED DIFFERENCES IN FIRST-PASS ALCOHOL METABOLISM IN MICE
SEX-RELATED AND STRAIN-RELATED DIFFERENCES IN FIRST-PASS ALCOHOL METABOLISM IN MICE
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DOI：
10.1016/0741-8329(94)00098-x
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发表时间：
1995-05-01
期刊：
影响因子：
2.3
通讯作者：
VERINA, D
中科院分区：
文献类型：
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作者：
DESROCHES, D;OREVILLO, C;VERINA, D
对三种品系的成年雄性和雌性小鼠C57 BL/10 J、C57 BL/6 J和DBA/2 J进行插管或腹腔内注射0.02 ml/g体重的25%乙醇溶液。30分钟后，测量他们的血液酒精水平（BAL）。另一组小鼠，包括两种性别，代表三种菌株，禁食12小时，并处死;他们的胃被删除，匀浆，并测定胃乙醇脱氢酶（GAD）的活性。与插管雄性动物相比，所有插管雌性动物的巴尔斯均较高。在注射组中观察到相反情况，显示雄性动物具有最高BAL值。GAD活性在3个品系的两性中均存在，且雄性最高。在插管组中存在明显的菌株相关差异，而在注射组中不存在。插管DBA动物具有最低的巴尔斯以及最高的GAD值。这些结果为小鼠的首过酒精代谢提供了证据，并显示了性别和应变对酒精胃氧化的影响。
Adult males and females of three strains of mice, C57BL/10J, C57BL/6J and DBA/2J, were intubated or injected intraperitoneally with 0.02 ml/g body weight of a 25% alcohol solution. Thirty minutes later, their blood alcohol levels (BAL) were measured. Another group of mice, including both sexes, representative of the three strains, was fasted for 12 h and sacrificed; their stomachs were removed, homogenized, and assayed for gastric alcohol dehydrogenase (GAD) activity. Higher BALs were found in all intubated females compared to the intubated males. The reverse was observed in the injected group, which showed the males with the highest BAL values. GAD activity was evidenced in both sexes of the three strains and it was highest in the males. Strain-related differences were evident in the intubated groups and not in the injected groups. Intubated DBA animals had the lowest BALs as well as the highest GAD values. The results provide evidence for first-pass alcohol metabolism in mice and show the effects of sex and strain on gastric oxidation of alcohol.