Production of succinate by recombinant Escherichia coli using acetate as the sole carbon source
Production of succinate by recombinant Escherichia coli using acetate as the sole carbon source
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DOI：
10.1007/s13205-018-1456-z
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发表时间：
2018-09-27
期刊：
影响因子：
2.8
通讯作者：
Li, Qiang
中科院分区：
文献类型：
--
作者：
Niu, Hao;Li, Ruirui;Li, Qiang
醋酸盐是一种潜在的低成本碳源，可以通过生物和化学过程产生。在本研究中，在野生型大肠杆菌MG 1655菌株中，缺失编码琥珀酸脱氢酶的sdhAB、编码异柠檬酸裂解酶调节剂的iclR和编码苹果酸酶的maeB，并过表达编码乙酰辅酶A合成酶的acs、编码柠檬酸合酶的gltA和编码乌头酸水合酶的acnB，产生重组E.大肠杆菌WCY-7菌株，该菌株能将乙酸转化为琥珀酸。在48小时分批发酵后，该菌株从50 mM乙酸钠积累11.23 mM琥珀酸。这项工作表明，微生物发酵使用乙酸作为唯一的碳源可能是一个合适的路线，以生产高产量的有价值的化学品。
Acetate is a potential low-cost carbon source that can be generated by biological and chemical processes. In this study, deletion of sdhAB encoding succinate dehydrogenase, iclR encoding the isocitrate lyase regulator, and maeB encoding the malic enzyme, and overexpression of acs encoding acetyl-CoA synthetase, gltA encoding citrate synthase, and acnB encoding aconitate hydratase in the wild-type Escherichia coli MG1655 strain yielded the recombinant E. coli strain WCY-7, which could synthesize succinate from acetate. After 48 h batch fermentation, this strain accumulated 11.23 mM succinate from 50 mM sodium acetate. This work indicates that microbial fermentation using acetate as the sole carbon source may be a suitable route to produce high yields of the valuable chemicals.