Taurine prevents glucose-induced lipid peroxidation and increased collagen production in cultured rat mesangial cells.
Taurine prevents glucose-induced lipid peroxidation and increased collagen production in cultured rat mesangial cells.
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牛磺酸可防止葡萄糖诱导的脂质过氧化并增加培养的大鼠系膜细胞中胶原蛋白的产生。
DOI：
10.1006/bbrc.1993.1282
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发表时间：
1993
影响因子：
3.1
通讯作者：
Robert S. Bienkowski
中科院分区：
文献类型：
--
作者：
Howard Trachtman;S. Futterweit;Robert S. Bienkowski
高血糖直接参与糖尿病肾病的发展。在多种培养细胞中，高葡萄糖浓度促进膜脂过氧化并刺激胶原蛋白的产生。牛磺酸是一种含硫氨基酸，是一种内源性抗氧化剂和抗纤维化剂。我们在体外测试了牛磺酸是否能改善环境葡萄糖升高对肾细胞的上述影响。通过丙二醛和偶联二烯含量评估，葡萄糖浓度从5.6提高到33.3 mM可增强大鼠系膜细胞的脂质过氧化作用，并使胶原生成增加59%。牛磺酸阻止了这两种葡萄糖诱导的系膜细胞效应。相比之下，无论是单独还是联合使用，高葡萄糖还是牛磺酸都不会影响来自肾小管上皮的MDCK或LLC-PK1细胞的脂质过氧化或胶原蛋白的产生。这些结果表明牛磺酸可能是一种有效的治疗糖尿病肾小球硬化的药物。
Hyperglycemia is directly involved in the development of diabetic nephropathy. A high glucose concentration promotes membrane lipid peroxidation and stimulates collagen production in a variety of cultured cells. Taurine, a sulfur amino acid, is an endogenous antioxidant and antifibrotic agent. We tested whether taurine ameliorates the above effects of elevated ambient glucose on renal cells in vitro. Raising glucose concentration from 5.6 to 33.3 mM enhanced lipid peroxidation in rat mesangial cells, as assessed by malondialdehyde and conjugated diene content, and increased collagen production by 59%. Taurine prevented both glucose-induced effects in mesangial cells. In contrast, neither high glucose nor taurine, alone or in combination, affected lipid peroxidation or collagen production in MDCK or LLC-PK1 cells, derived from renal tubular epithelium. These results indicate that taurine may be a useful therapeutic agent to attenuate diabetic glomerulosclerosis.