ISOLATION OF A COMPLEMENTARY-DNA THAT ENCODES THE MAMMALIAN SPLICING FACTOR SC35
ISOLATION OF A COMPLEMENTARY-DNA THAT ENCODES THE MAMMALIAN SPLICING FACTOR SC35
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DOI：
10.1126/science.1373910
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发表时间：
1992-04-24
期刊：
影响因子：
56.9
通讯作者：
MANIATIS, T
中科院分区：
文献类型：
--
作者：
FU, XD;MANIATIS, T
哺乳动物剪接因子SC35是剪接反应的第一步和剪接体组装所必需的。编码该蛋白的互补DNA的克隆和鉴定表明，它是包括哺乳动物SF2/ASF在内的剪接因子家族的成员。这个蛋白质家族的特征是存在一个核糖核蛋白(RNP)型RNA结合基序和一个羧基末端富含丝氨酸-精氨酸(SR)结构域。对DNA序列数据库的检索发现，原癌基因c-myb的胸腺特异性外显子(E(T))编码在SC35基因的反义链上。
The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly. The cloning and characterization of a complementary DNA encoding this protein revealed that it is a member of a family of splicing factors that includes mammalian SF2/ASF. This family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal serine-arginine-rich (SR) domain. A search of the DNA sequence database revealed that the thymus-specific exon (E(T)) of the c-myb proto-oncogene is encoded on the antisense strand of the SC35 gene.