The ryanodine receptor/calcium channel genes are widely and differentially expressed in murine brain and peripheral tissues.
The ryanodine receptor/calcium channel genes are widely and differentially expressed in murine brain and peripheral tissues.
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DOI：
10.1083/jcb.128.5.893
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发表时间：
1995-03
期刊：
影响因子：
--
通讯作者：
Sorrentino V
中科院分区：
文献类型：
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作者：
Giannini G;Conti A;Mammarella S;Scrobogna M;Sorrentino V
Ryanodine受体(RyRs)是细胞内钙释放通道，参与控制细胞内钙水平。与可能普遍存在的由1，4，5-三磷酸肌醇操控的钙通道(1，4，5-三磷酸受体)不同，RyRs主要被认为是控制骨骼肌和心肌收缩的钙释放通道。最近越来越多的证据表明，RyRs可能被更广泛地表达，但这一点从未被广泛研究过。因此，我们克隆了三个与小鼠RyR同源物相对应的cDNA，以全面分析它们在小鼠组织中的表达。在这里，我们报告了这三个基因在几乎所有被分析的组织中都有表达，其中观察到了组织特有的表达模式。在子宫和输精管中，RyR3的表达定位于这些器官的平滑肌成分。在睾丸中，RyR1和RyR3在生殖细胞中有表达。在体外培养的细胞系中也检测到RyR mRNAs。RyR1、RyR2和RyR3mRNA在大脑和小脑中均有表达。原位分析揭示了中枢神经系统不同区域中特定细胞类型的表达模式。三种兰尼定受体基因在中枢神经系统中的差异表达也被用针对各自蛋白质的特异性抗体证实。这种广泛的表达模式表明，RyRs可能参与了细胞内钙稳态的调节，其范围比先前认识的更广泛。
Ryanodine receptors (RyRs) are intracellular calcium release channels that participate in controlling cytosolic calcium levels. At variance with the probably ubiquitous inositol 1,4,5-trisphosphate-operated calcium channels (1,4,5-trisphosphate receptors), RyRs have been mainly regarded as the calcium release channels controlling skeletal and cardiac muscle contraction. Increasing evidence has recently suggested that RyRs may be more widely expressed, but this has never been extensively examined. Therefore, we cloned three cDNAs corresponding to murine RyR homologues to carry a comprehensive analysis of their expression in murine tissues. Here, we report that the three genes are expressed in almost all tissues analyzed, where tissue-specific patterns of expression were observed. In the uterus and vas deferens, expression of RyR3 was localized to the smooth muscle component of these organs. In the testis, expression of RyR1 and RyR3 was detected in germ cells. RyR mRNAs were also detected in in vitro-cultured cell lines. RyR1, RyR2, and RyR3 mRNA were detected in the cerebrum and in the cerebellum. In situ analysis revealed a cell type-specific pattern of expression in the different regions of the central nervous system. The differential expression of the three ryanodine receptor genes in the central nervous system was also confirmed using specific antibodies against the respective proteins. This widespread pattern of expression suggests that RyRs may participate in the regulation of intracellular calcium homeostasis in a range of cells wider than previously recognized.