Dietary nano‐selenium enhances antioxidant capacity and hypoxia tolerance of grass carp Ctenopharyngodon idella fed with high‐fat diet
Dietary nano‐selenium enhances antioxidant capacity and hypoxia tolerance of grass carp Ctenopharyngodon idella fed with high‐fat diet
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DOI：
10.1111/anu.13016
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发表时间：
2020-04
影响因子：
3.5
通讯作者：
Haibo Yu;Cheng Zhang;Xiaotian Zhang;Chi Wang;Pengju Li;Guohao Liu;Xianfang Yan;Xiaofan Xiong;Li Zhang;Jingyao Hou;Sha Liu;Jishu Zhou;H. Ji
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文献类型：
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作者：
Haibo Yu;Cheng Zhang;Xiaotian Zhang;Chi Wang;Pengju Li;Guohao Liu;Xianfang Yan;Xiaofan Xiong;Li Zhang;Jingyao Hou;Sha Liu;Jishu Zhou;H. Ji
为评价饲粮中添加纳米硒对草鱼高脂饲料抗氧化能力和耐缺氧能力的影响，实验鱼分别以0（对照）、0.3、0.6、0.9和1.2 mg/kg的纳米硒添加量饲喂10周。饲喂试验结束后，对部分鱼进行缺氧应激处理。结果表明，0.6和0.9 mg/kg纳米硒组草鱼存活率显著提高，血清谷丙转氨酶（ALT）和天冬氨酸转氨酶（AST）含量显著低于对照组。此外，饲粮中添加0.3和0.6 mg/kg纳米硒显著提高了鱼的谷胱甘肽过氧化物酶（GPX）活性，降低了丙二醛（MDA）含量。饲粮纳米硒通过促进肝胰腺中NF - E2相关核因子2 (Nrf2) mRNA的表达，显著提高了GPX1和过氧化氢酶（CAT） mRNA的表达。低氧胁迫后，添加0.3和0.6 mg/kg纳米硒的鱼体内GPX和超氧化物歧化酶（SOD）活性显著增强，MDA含量降低，死亡率降低。综上所述，饲粮中添加适量纳米硒可提高草鱼的抗氧化能力和耐缺氧能力。
To evaluate the effects of dietary nano‐selenium (Nano‐Se) on antioxidant capacity and hypoxia tolerance of grass carp fed with high‐fat diet, experimental fishes were fed Nano‐Se supplemented diets at doses of 0 (Control), 0.3, 0.6, 0.9 and 1.2 mg/kg for 10 weeks. After feeding trial, a part of the fishes were exposed to hypoxia stress. Results showed that the survival ratio of grass carp significantly increased in 0.6 and 0.9 mg/kg Nano‐Se group, and the content of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) significantly decreased in 0.6–1.2 mg/kg Nano‐Se groups compared with the control group. In addition, dietary Nano‐Se significantly enhanced glutathione peroxidase (GPX) activity and reduced the malondialdehyde (MDA) content in fishes fed diets with 0.3 and 0.6 mg/kg Nano‐Se. Dietary Nano‐Se significantly elevated mRNA expression of GPX1 and catalase (CAT) by promoting the mRNA expression of NF‐E2‐related nuclear factor 2 (Nrf2) in the hepatopancreas. After hypoxia stress, the GPX and superoxide dismutase (SOD) activities were significantly enhanced, and the MDA content and mortality rate consequently decreased in fishes fed diets with 0.3 and 0.6 mg/kg Nano‐Se. In summary, these results suggested that optimal Nano‐Se in diet enhanced the antioxidant capacity and hypoxia tolerance of grass carp.