Omics-Based Comparative Transcriptional Profiling of Two Contrasting Rice Genotypes during Early Infestation by Small Brown Planthopper.
Omics-Based Comparative Transcriptional Profiling of Two Contrasting Rice Genotypes during Early Infestation by Small Brown Planthopper.
复制标题
基于组学的两种对比水稻基因型在小褐飞虱早期侵染期间的比较转录谱
DOI：
10.3390/ijms161226128
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发表时间：
2015-12-03
影响因子：
5.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Zhang W;Yang L;Li M;Ma B;Yan C;Chen J
褐飞虱是水稻的主要害虫之一。虽然不同的生物化学途径，参与水稻响应稻飞虱侵扰已被记录，目前还不清楚哪些个别的代谢途径是响应稻飞虱侵扰。在这项研究中，一个组学为基础的比较转录谱的两个对比水稻基因型，SBPH抗性和SBPH敏感的水稻品系，水稻个体代谢途径响应SBPH侵染进行了评估。在SBPH胁迫下，166条代谢途径受到不同程度的调控，其中超过1/3的代谢途径在两种水稻基因型间表现出相似的变化模式，两种水稻基因型间变化模式的差异主要表现在生物合成途径上，能量代谢途径的差异最为明显。将Pathway Tools Omics Viewer与网络工具Venn相结合，分别确定了21条和6条可能与SBPH耐药和易感性相关的代谢途径。本研究建立了一个基于组学的水稻抗虫和感虫植株在虫害早期的比较转录谱，为研究水稻-昆虫互作提供了重要信息。这些结果将从生物化学途径的角度提供深入了解水稻植物如何应对SBPH的早期侵染。
The small brown planthopper (SBPH) is one of the destructive pests of rice. Although different biochemical pathways that are involved in rice responding to planthopper infestation have been documented, it is unclear which individual metabolic pathways are responsive to planthopper infestation. In this study, an omics-based comparative transcriptional profiling of two contrasting rice genotypes, an SBPH-resistant and an SBPH-susceptible rice line, was assessed for rice individual metabolic pathways responsive to SBPH infestation. When exposed to SBPH, 166 metabolic pathways were differentially regulated; of these, more than one-third of metabolic pathways displayed similar change patterns between these two contrasting rice genotypes; the difference of change pattern between these two contrasting rice genotypes mostly lies in biosynthetic pathways and the obvious difference of change pattern lies in energy metabolism pathways. Combining the Pathway Tools Omics Viewer with the web tool Venn, 21 and 6 metabolic pathways which potentially associated with SBPH resistance and susceptibility, respectively were identified. This study presents an omics-based comparative transcriptional profiling of SBPH-resistant and SBPH-susceptible rice plants during early infestation by SBPH, which will be very informative in studying rice-insect interaction. The results will provide insight into how rice plants respond to early infestation by SBPH from the biochemical pathways perspective.