The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells.
The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells.
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DOI：
10.1016/j.bbrc.2011.10.007
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发表时间：
2011-11
影响因子：
3.1
通讯作者：
Yui Watabe;Y. Baba;H. Nakauchi;A. Mizota;Sumiko Watanabe
中科院分区：
文献类型：
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作者：
Yui Watabe;Y. Baba;H. Nakauchi;A. Mizota;Sumiko Watanabe
锌指转录因子Zic家族的成员在多种发育过程中起着关键作用。使用DNA微阵列分析，我们发现Zics在小鼠视网膜周边区域的SSEA-1阳性早期视网膜祖细胞中强烈表达。逆转录聚合酶链反应，从视网膜的mRNA在不同的发展阶段表明，Zic 1和Zic 2的表达在胚胎视网膜，然后逐渐消失在视网膜发育过程中。Zic 3也在胚胎视网膜中表达;其表达水平略有下降，但直到成年才表达。我们在胚胎17.5天的视网膜祖细胞中过表达Zic 1、Zic 2或Zic 3，并将视网膜作为外植体培养2周。视杆光感受器的数量比对照少，但没有其他细胞类型显示对照和Zic过表达细胞之间的显着差异。正常视网膜前体细胞的增殖活性在培养5天后降低，如在正常体内发育过程中观察到的。然而，表达Zic的视网膜细胞在第5天和第7天继续增殖，表明Zic维持视网膜祖细胞的增殖活性。由于Zic 1、2和3的作用在视网膜祖细胞的分化和增殖方面是不可区分的，因此建议Zic在视网膜发育中的冗余功能。
Members of the Zic family of zinc finger transcription factors play critical roles in a variety of developmental processes. Using DNA microarray analysis, we found that Zics are strongly expressed in SSEA-1-positive early retinal progenitors in the peripheral region of the mouse retina. Reverse-transcription polymerase chain reaction using mRNA from the retina at various developmental stages showed that Zic1 and Zic2 are expressed in the embryonic retina and then gradually disappear during retinal development. Zic3 is also expressed in the embryonic retina; its expression level slightly decreases but it is expressed until adulthood. We overexpressed Zic1, Zic2, or Zic3 in retinal progenitors at embryonic day 17.5 and cultured the retina as explants for 2weeks. The number of rod photoreceptors was fewer than in the control, but no other cell types showed significant differences between control and Zic overexpressing cells. The proliferation activity of normal retinal progenitors decreased after 5days in culture, as observed in normal in vivo developmental processes. However, Zic expressing retinal cells continued to proliferate at days 5 and 7, suggesting that Zics sustain the proliferation activities of retinal progenitor cells. Since the effects of Zic1, 2, and 3 are indistinguishable in terms of differentiation and proliferation of retinal progenitors, the redundant function of Zics in retinal development is suggested.