Antioxidant and cytoprotective activities of extracts prepared from fruit and vegetable wastes and by-products
Antioxidant and cytoprotective activities of extracts prepared from fruit and vegetable wastes and by-products
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DOI：
10.1016/j.foodchem.2014.06.099
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发表时间：
2015-01-15
期刊：
影响因子：
8.8
通讯作者：
Nakamura, Soichiro
中科院分区：
文献类型：
--
作者：
Kabir, Faisal;Tow, Wei Wei;Nakamura, Soichiro
在这项研究中，测试了水果和蔬菜废物及其副产品的多酚含量及其抗氧化活性。通过 Folin-Ciocalteu 测定评估，多酚含量最高的是葡萄籽的热水提取物，其次是荞麦壳的乙醇提取物。通过 2,2-二苯基-1-三硝基苯肼 (DPPH) 测定，葡萄籽的热水提取物中也检测到最高的抗氧化活性，其次是未成熟李子的乙醇提取物。大多数样品对非洲猴肾 (MA 104) 细胞中 2,2'-偶氮双-(2-脒基丙烷)二盐酸盐 (AAPH) 过氧自由基产生剂诱导的氧化应激具有保护作用。含有大量酚类物质（超过 30 mg ChAE/g）的样品通常表现出较高的抗氧化活性，并对 AAPH 诱导的氧化应激具有保护作用。这项研究表明，水果和蔬菜废物及副产品是大量具有抗氧化特性的酚类物质的良好来源。 (C) 2014 Elsevier Ltd. 保留所有权利。
In this study, fruit and vegetable wastes and by-products were tested for polyphenol content and their antioxidant activity. The highest content of polyphenols as assessed by the Folin-Ciocalteu assay was the hot-water extract of grape seed, followed by the ethanol extract of buckwheat hull. The highest antioxidant activity measured by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays was also detected in the hotwater extract of grape seed, followed by the ethanol extract of immature prune. Most of samples showed protective effects against oxidative stress induced by 2,2'-azobis-(2-amidinopropane) dihydrochloride (AAPH) peroxyl radical generator in African monkey kidney (MA 104) cells. Samples containing high amounts of phenolics (more than 30 mg ChAE/g) generally showed high antioxidant activity and a protective effect against AAPH-induced oxidative stress. This study demonstrates that fruit and vegetable wastes and by-products are good sources of high amounts of phenolics with antioxidant properties. (C) 2014 Elsevier Ltd. All rights reserved.