THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
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THAP11 通过调节 c-Myc 信号通路发挥胃癌肿瘤抑制剂的作用
DOI：
10.1155/2020/7838924
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发表时间：
2020-08
影响因子：
--
通讯作者：
Han Jiming
中科院分区：
文献类型：
--
作者：
Zhang Jing;Zhang Huahua;Shi Haiyan;Wang Fenghui;Du Juan;Wang Yu;Wei Yameng;Xue Wanjuan;Li Dan;Feng Yun;Yan Jing;Gao Yi;Li Jia;Han Jiming
我们的目的是研究THAP11 （thanatos-associated protein - 11）在胃癌中的作用及其调控机制。采用qRT-PCR和Western blot分析51对胃癌组织及相应癌旁组织中THAP11的表达。THAP11过表达或敲低后，在MKN-45细胞中检测细胞增殖、细胞周期和细胞凋亡。我们发现THAP11在GC组织和GC细胞系中显著下调。功能上，THAP11过表达可显著抑制细胞生长，诱导G1/G0细胞周期阻滞，促进MKN-45细胞凋亡，而THAP11沉默可促进细胞生长，增加DNA合成，抑制细胞凋亡。此外，THAP11负性调控c-Myc的表达，降低cyclinD1蛋白表达，增加p27和p21蛋白表达水平。我们还发现THAP11诱导的细胞生长抑制通过c-Myc过表达得以恢复，进一步证实THAP11通过c-Myc途径抑制胃癌细胞生长。THAP11在胃癌中作为细胞生长抑制因子，可能通过负调控c-Myc通路发挥作用。
We aim to investigate the role of THAP11 (thanatos-associated protein11) in gastric cancer and its regulation mechanisms. THAP11 expression was analyzed in 51 pairs of GC tissues and the corresponding paracancerous tissues by qRT-PCR and Western blot. After THAP11 was overexpressed or knocked-down, cell proliferation, cell cycle, and apoptosis were detected in MKN-45 cells. We found that THAP11 was significantly downregulated in GC tissues and GC cell lines. Functionally, THAP11 overexpression markedly inhibited cell growth, induced G1/G0 cell-cycle arrest, and promoted cell apoptosis of MKN-45 cells, while silencing of THAP11 led to increased cell growth, increased DNA synthesis, and inhibited apoptosis. In addition, THAP11 negatively regulated the expression of c-Myc, decreased cyclinD1 protein, and increased p27 and p21 protein levels. We also found cell growth suppression induced by THAP11 was rescued by c-Myc overexpression, further confirming that THAP11 suppresses gastric cancer cell growth via the c-Myc pathway. THAP11 acts as a cell growth suppressor and exerts its role possibly through negatively regulating c-Myc pathway in gastric cancer.
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影响因子：
1.4
作者：
W. Zeng;Han-ying Sun;Fankai Meng;Zeming Liu;J. Xiong;Sheng Zhou;Fan Li;Jia Hu;Zhiquan Hu;Zheng Liu
通讯作者：
W. Zeng;Han-ying Sun;Fankai Meng;Zeming Liu;J. Xiong;Sheng Zhou;Fan Li;Jia Hu;Zhiquan Hu;Zheng Liu
影响因子：
3.1
作者：
Alev Richter;R. Hollstein;Eva Hebert;F. Vulinovic;Juliane Eckhold;Alma Osmanovic;R. Depping;F. Kaiser;K. Lohmann
通讯作者：
Alev Richter;R. Hollstein;Eva Hebert;F. Vulinovic;Juliane Eckhold;Alma Osmanovic;R. Depping;F. Kaiser;K. Lohmann
影响因子：
64.5
作者：
M. Déjosez;J. Krumenacker;L. Zitur;M. Passeri;Li-Fang Chu;Songyang Zhou;J. Thomson;T. Zwaka
通讯作者：
M. Déjosez;J. Krumenacker;L. Zitur;M. Passeri;Li-Fang Chu;Songyang Zhou;J. Thomson;T. Zwaka
影响因子：
5.3
作者：
Parker, J. Brandon;Palchaudhuri, Santanu;Chakravarti, Debabrata
通讯作者：
Chakravarti, Debabrata
影响因子：
3.5
作者：
Pandey, N;Mittal, U;Mukerji, M
通讯作者：
Mukerji, M