Effects of camptothecin on the rice blast fungus Magnaporthe oryzae
Effects of camptothecin on the rice blast fungus Magnaporthe oryzae
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DOI：
10.1016/j.pestbp.2019.11.002
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发表时间：
2020-02-01
影响因子：
4.7
通讯作者：
Feng, Xu
中科院分区：
文献类型：
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作者：
Xu, Shu;Wang, Bi;Feng, Xu
稻瘟病（Magnaporthe oryzae B. Couch）是水稻最具破坏性的病害之一。喜树碱（Camptothecin， CPT）主要从喜树（Camptotheca acuminata）中分离得到，具有良好的抗肿瘤活性，是一种潜在的生物农药。我们对CPT对11种真菌、3种卵菌和4种细菌的抑菌活性进行了研究，发现CPT对M. oryzae具有较强的抑菌活性，为开发稻瘟病杀菌剂奠定了基础。然而，CPT对米曲菌的抗真菌作用有待进一步研究。本研究进一步研究了CPT对M. oryzae的抗真菌活性，结果表明CPT对M. oryzae在体内和体外均有效。对CPT处理后M. oryzae的转录组进行了分析，结果表明CPT对M. oryzae的“翻译”和“碳水化合物代谢/能量代谢”具有较强的抑制作用。通过对M. oryzae生理特性的分析，证实了CPT对M. oryzae的RNA合成、蛋白质合成和碳水化合物/能量代谢的抑制作用，并对M. oryzae膜造成损伤。分子模拟结果表明，CPT结合到M. oryzae dna -拓扑异构酶I复合物的界面上。综上所述，CPT是开发稻瘟病杀菌剂的良好先导剂。CPT可能与m.o ryzae的dna -拓扑异构酶I复合物结合，从而影响“翻译”和“碳水化合物代谢/能量代谢”，导致细胞死亡。
Rice blast caused by Magnaporthe oryzae B. Couch is one of the most devastating diseases on rice. Camptothecin (CPT), which was primarily isolated from Camptotheca acuminata, is well-known for its anti-tumor activities, and is also developed as a potential biological pesticide. We previously investigated the anti-microbial activities of CPT against 11 fungi, 3 oomycetes, and 4 bacteria, and found that CPT was strongly effective against M. oryzae, indicating its potential as a lead for developing fungicide against rice blast. However, the anti-fungal effects of CPT on M oryzae need further elucidation. In this study, the anti-fungal activities of CPT against M oryzae were further investigated, which revealed that CPT was effective against M. oryzae both in vitro and in vivo. The transcriptome of M. oryzae was analyzed after CPT treatment, which showed that CPT had a strong inhibitory effect on 'translation' and 'carbohydrate metabolism/energy metabolism' of M. oryzae. Some physiology characteristics of M. oryzae were also assayed, which confirmed that CPT inhibited RNA synthesis, protein synthesis, and carbohydrate metabolism/energy metabolism of M. oryzae, and caused membrane damage. The molecular simulation result showed that CPT binds to the interface of DNA-topoisomerase I complex of M. oryzae. In conclusion, CPT is a promising lead for developing fungicide against rice blast. CPT may bind to DNA-topoisomerase I complex of M. oryzae, thus affecting 'translation' and 'carbohydrate metabolism/energy metabolism', leading to cell death.