Genoarchitecture of the extended amygdala in zebra finch, and expression of FoxP2 in cell corridors of different genetic profile.
Genoarchitecture of the extended amygdala in zebra finch, and expression of FoxP2 in cell corridors of different genetic profile.
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DOI：
10.1007/s00429-016-1229-6
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发表时间：
2017-01
影响因子：
3.1
通讯作者：
Medina L
中科院分区：
文献类型：
--
作者：
Vicario A;Mendoza E;Abellán A;Scharff C;Medina L
我们使用一组编码转录因子（Pax6，Islet 1，Nkx2.1，Lhx6，Lhx5，Lhx9，FoxP2）和神经肽的基因来研究发育中的斑胸草雀的延伸杏仁核。我们确定了中央延伸杏仁核的不同组成部分，与小鼠和鸡中发现的组成部分相当，包括嵌入杏仁核细胞、中央杏仁核和终纹外侧床核。许多细胞可能起源于背侧纹状体区、腹侧纹状体区或苍白球区，如小鼠和鸡的情况。此外，中央延伸杏仁核的细胞亚群似乎起源于丘脑前隆起。作为一般原则，这些具有特定遗传特征和胚胎起源的不同细胞沿着延伸的杏仁核形成单独或部分混合的细胞走廊，这些细胞走廊可能参与不同的功能途径。此外，我们还鉴定了斑胸草雀的内侧杏仁核。与鸡和小鼠一样，它位于软腭下区，富含苍白球视前区起源的细胞，含有来自腹侧软腭、下丘脑腹侧和丘脑前隆起的少量移民细胞亚群。我们还提出，终纹的内侧床核是由几个平行的细胞走廊具有不同的遗传配置文件和胚胎起源：视前，苍白球，下丘脑，和丘脑前。这些具有不同起源的细胞通道中的几个表达FoxP2，FoxP2是一种与突触可塑性有关的转录因子。我们的研究结果为利用斑胸草雀了解社会行为的神经基础（其中涉及扩展杏仁核）的研究铺平了道路。
We used a battery of genes encoding transcription factors (Pax6, Islet1, Nkx2.1, Lhx6, Lhx5, Lhx9, FoxP2) and neuropeptides to study the extended amygdala in developing zebra finches. We identified different components of the central extended amygdala comparable to those found in mice and chickens, including the intercalated amygdalar cells, the central amygdala, and the lateral bed nucleus of the stria terminalis. Many cells likely originate in the dorsal striatal domain, ventral striatal domain, or the pallidal domain, as is the case in mice and chickens. Moreover, a cell subpopulation of the central extended amygdala appears to originate in the prethalamic eminence. As a general principle, these different cells with specific genetic profiles and embryonic origin form separate or partially intermingled cell corridors along the extended amygdala, which may be involved in different functional pathways. In addition, we identified the medial amygdala of the zebra finch. Like in the chickens and mice, it is located in the subpallium and is rich in cells of pallido-preoptic origin, containing minor subpopulations of immigrant cells from the ventral pallium, alar hypothalamus and prethalamic eminence. We also proposed that the medial bed nucleus of the stria terminalis is composed of several parallel cell corridors with different genetic profile and embryonic origin: preoptic, pallidal, hypothalamic, and prethalamic. Several of these cell corridors with distinct origin express FoxP2, a transcription factor implicated in synaptic plasticity. Our results pave the way for studies using zebra finches to understand the neural basis of social behavior, in which the extended amygdala is involved.