Plasma matrix metalloproteinase-9 response to downhill running in humans.
Plasma matrix metalloproteinase-9 response to downhill running in humans.
复制标题
血浆基质金属蛋白酶 9 对人类下坡跑的反应。
DOI：
10.1055/s-0033-1353212
复制
发表时间：
2014
影响因子：
2.5
通讯作者：
Byrnes,WC
中科院分区：
文献类型：
--
作者：
Welsh,MC;Allen,DL;Byrnes,WC
基质金属蛋白酶-9是一种能够降解肌肉细胞外基质蛋白的蛋白水解酶。MMP-9或其抑制剂金属蛋白酶组织抑制剂-1（TIMP-1）的全身水平有可能作为运动性肌肉损伤的血液标志物。本研究的目的是确定偏心主导的任务，下坡跑（DHR），是否产生血浆MMP-9或TIMP-1的变化，并检查MMP-9/TIMP-1水平和肌肉损伤的间接指标之间的关系。受试者久坐不动（SED，n=12）或有集中性偏差训练史（CON，n=9）。在DHR前（Ex前）、DHR后即刻（Ex后）和DHR后1、2、4和7天（−10°）测量MMP-9和TIMP-1，并与不适评分、肌酸激酶活性和力量损失进行比较。在Ex后1天，不适增加（5.6±7.8至45.5±19.9 mm; 0-100 mm量表），力量降低（−6.9±1.6%），CK增加（162.9±177.2%）。MMP-9在CONC和SED中在Ex后（32.7±33.6%）和在SED中在4天（66.9±88.1%）适度但显著地增加。然而，个体反应是可变的。MMPs与不适评分、血浆CK或力量之间无相关性。虽然血浆MMP-9的变化可以在DHR后的体循环中检测到，但它们很小，并且不对应于其他损伤标志物。
Matrix metalloproteinase-9 is a proteolytic enzyme capable of degrading proteins of the muscle extracellular matrix. Systemic levels of MMP-9 or its inhibitor, tissue inhibitor of metalloproteinase-1 (TIMP-1), have the potential to serve as blood markers of exercise-induced muscle damage. The purpose of this study was to determine if an eccentrically-dominated task, downhill running (DHR), produces changes in plasma MMP-9 or TIMP-1 and examine the relationship between MMP-9/TIMP-1 levels and indirect indicators of muscle damage. Subjects were sedentary (SED, n=12) or had a history of concentrically-biased training (CON, n=9). MMP-9 and TIMP-1 were measured before (Pre-Ex), immediately after (Post-Ex), and 1-, 2-, 4-, and 7-days post-DHR (−10°), and compared to discomfort ratings, creatine kinase activity and strength loss. At 1-day Post-Ex, discomfort increased (5.6±7.8 to 45.5±19.9 mm; 0–100 mm scale), strength decreased (−6.9±1.6%) and CK increased (162.9±177.2%). MMP-9 was modestly but significantly increased at Post-Ex in both CONC and SED (32.7±33.6%) and at 4-days in SED (66.9±88.1%), Individual responses were variable, however. There were no correlations between MMPs and discomfort ratings, plasma CK or strength. While plasma MMP-9 changes may be detectable in the systemic circulation after DHR, they are small and do not correspond to other markers of damage.