Geographic origin of Explanatum explanatum (Creplin, 1847) Fukui, 1929 detected from domestic water buffaloes in Sri Lanka
Geographic origin of Explanatum explanatum (Creplin, 1847) Fukui, 1929 detected from domestic water buffaloes in Sri Lanka
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Explanatum explanatum (Creplin, 1847) 福井，1929 年从斯里兰卡家养水牛中检测到的地理起源
DOI：
10.1016/j.meegid.2021.104806
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Ichikawa-Seki Madoka
中科院分区：
文献类型：
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作者：
Thang Tran Nhat;Sivakumar Thillaiampalam;Kothalawala Hemal;Silva Seekkuge Susil Priyantha;Yokoyama Naoaki;Ichikawa-Seki Madoka
Explanatum explanatum 的成年阶段对于反刍动物，特别是水牛的生产具有经济重要性。该物种在印度次大陆已被广泛报道。最近，在孟加拉国、尼泊尔和印度进行了分子分析，揭示了该物种的传播途径。在本研究中，我们重点关注E。斯里兰卡分布有解释性吸虫。从斯里兰卡水牛身上采集了52只吸虫，鉴定为E。解释基于核核糖体 DNA 的内部转录间隔区 2 (ITS2) 区域。对 DNA 样本中线粒体 NADH 脱氢酶亚基 1 (nad1) 基因的分析检测到 18 个单倍型，其中 5 个与 IndianE 的单倍型相同。解释。成对注视指数值表明，斯里兰卡人口与印度人口的关系比与孟加拉国人口或尼泊尔人口的关系更为密切。斯里兰卡人口的遗传变异性明显低于印度人口，这表明印度人口是斯里兰卡人口的祖先。包括水牛在内的宿主反刍动物的移动可能与吸虫传入斯里兰卡有关。我们的研究结果为阐明大肠杆菌的地理起源提供了有用的信息。解释分布于印度次大陆。
The adult stage ofExplanatum explanatumhas economic importance in the production of ruminants, especially water buffaloes. This species has been widely reported in the Indian sub-continent. Recently, molecular analyses to reveal the dispersal route of this species were performed in Bangladesh, Nepal, and India. In the present study, we focused onE. explanatumdistributed in Sri Lanka.A total of 52 flukes were collected from water buffaloes in Sri Lanka and identified asE. explanatumbased on the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA. Analysis of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene from DNA samples detected 18 haplotypes, and five of them were identical to those from the IndianE. explanatum. The pairwise fixation index value indicated that the Sri Lankan population had a comparatively closer relationship with the Indian population than with the Bangladeshi or Nepalese populations. The Sri Lankan population showed significantly lower genetic variability than the Indian population, suggesting that the Indian population was the ancestor of the Sri Lankan population. The movement of host ruminants, including water buffaloes, was probably involved in the introduction of the fluke into Sri Lanka. The results of our study provide useful information for elucidating the geographic origin ofE. explanatumdistributed in the Indian subcontinent.