Orally administered epigallocatechin gallate attenuates light-induced photoreceptor damage
Orally administered epigallocatechin gallate attenuates light-induced photoreceptor damage
复制标题
DOI：
10.1016/j.brainresbull.2008.01.022
复制
发表时间：
2008-07-01
影响因子：
3.8
通讯作者：
Osborne, Neville N.
中科院分区：
文献类型：
--
作者：
da Silveira Andrade da Costa, Belmira Lara;Fawcett, Rebecca;Osborne, Neville N.
EGCG是绿茶的主要成分，它有许多特性，包括它是一种强大的抗氧化剂。本研究的目的是推断在白化大鼠的饮用水中加入EGCG是否会减弱轻度损伤（2200ix， 24小时）对视网膜的影响。在视网膜外核层检测到tunel阳性细胞，表明紧致损伤诱导光感受器变性的有效性。此外，位于光感受器的Ret-P1和视紫红质mRNA也显著减少，视网膜电图的a波和b波振幅也减少，表明光感受器特别受光的影响。光引起的主要位于Muller细胞的GFAP蛋白/mRNA的增加表明其他视网膜成分也受到光损伤的影响。然而，与双相细胞（α - pkc）、神经节细胞（y-1）和无毛细胞（GABA）相关的抗原却未受影响。光损伤还引起与凋亡有关的多种蛋白（caspase-3、caspase-8、PARP、Bad和Bcl-2）含量的变化。当饮用EGCG时，一些由轻微损伤引起的视网膜变化明显减弱。光引起的a波和b波和光感受器特异性mrna /蛋白的减少明显较少。此外，EGCG可以减弱光对某些凋亡蛋白的影响（尤其是在2天后），但对光诱导的GFAP蛋白/mRNA的上调似乎没有显著影响。由此得出结论，口服EGCG可以减弱光对白化大鼠视网膜的有害影响，其中光感受器主要受到影响。(c) 2008爱思唯尔公司版权所有。
EGCG, a major component of green tea, has a number of properties which includes it being a powerful antioxidant. The purpose of this investigation was to deduce whether inclusion of EGCG in the drinking water of albino rats attenuates the effect of a light insult (2200 Ix, for 24 h) to the retina. TUNEL-positive cells were detected in the outer nuclear layer of the retina, indicating the efficacy of the tight insult in inducing photoreceptor degeneration. Moreover, Ret-P1 and the mRNA for rhodopsin located at photoreceptors were also significantly reduced as well as the amplitude of both the a- and b-waves of the electroretinogram was also reduced showing that photoreceptors in particular are affected by light. An increase in protein/mRNA of GFAP located primarily to Muller cells caused by light shows that other retinal components are also influenced by the light insult. However, antigens associated with bipolar (alpha-PKC), ganglion (Thy-1) and amacrine (GABA) cells, in contrast, appeared unaffected. The light insult also caused a change in the content of various proteins (caspase-3, caspase-8, PARP, Bad, and Bcl-2) involved in apoptosis. A number of the changes to the retina caused by a light insult were significantly attenuated when EGCG was in the drinking water. The reduction of the a- and b-waves and photoreceptor specific mRNAs/protein caused by light were significantly less. In addition, EGCG attenuated the changes caused by light to certain apoptotic proteins (especially at after 2 days) but did not appear to significantly influence the light-induced up-regulation of GFAP protein/mRNA. It is concluded that orally administered EGCG blunts the detrimental effect of light to the retina of albino rats where the photoreceptors are primarily affected. (c) 2008 Elsevier Inc. All rights reserved.