The Campoletis sonorensis ichnovirus vankyrin protein P-vank-1 inhibits apoptosis in insect Sf9 cells
The Campoletis sonorensis ichnovirus vankyrin protein P-vank-1 inhibits apoptosis in insect Sf9 cells
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DOI：
10.1111/j.1365-2583.2009.00892.x
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发表时间：
2009-08-01
影响因子：
2.6
通讯作者：
Webb, B. A.
中科院分区：
文献类型：
--
作者：
Fath-Goodin, A.;Kroemer, J. A.;Webb, B. A.
Campoletis sonorensis ichnovirus（CsIV）vankyrin基因编码含有截短的锚蛋白重复结构域的蛋白质，所述截短的锚蛋白重复结构域与NF-κ B转录因子抑制剂I κ B的抑制结构域具有序列同源性。CsIV vankyrin蛋白被认为在寄生宿主的免疫应答和/或发育事件期间参与NF-κ B活性的抑制。在这里，我们报告说，当P-vank-1从Sf 9细胞中稳定表达时，杆状病毒感染，紫外线照射，并与未转化的Sf 9细胞相比，诱导凋亡的化学喜树碱治疗后，观察到这些细胞的存活时间延长。此外，P-vank-1抑制稳定表达P-vank-1的Sf 9细胞诱导凋亡后的核和核小体间降解以及caspase活性。这是第一次报道具有抗凋亡功能的多DNA病毒蛋白。
The Campoletis sonorensis ichnovirus (CsIV) vankyrin genes encode proteins containing truncated ankyrin repeat domains with sequence homology to the inhibitory domains of NF-kappa B transcription factor inhibitors, I kappa Bs. The CsIV vankyrin proteins are thought to be involved in the suppression of NF-kappa B activity during immune response and/or developmental events in the parasitized host. Here we report that when P-vank-1 was expressed stably from Sf9 cells, prolonged survival of these cells was observed after baculovirus infection, UV irradiation, and treatment with the apoptosis-inducing chemical camptothecin compared to untransformed Sf9 cells. Furthermore, P-vank-1 inhibited nuclear and internucleosomal degradation and caspase activity after induction of apoptosis in Sf9 cells stably expressing P-vank-1. This is the first report of a polydnavirus protein with anti-apoptotic function.