Intracisternal injection of TRH antibody blocks gastric emptying stimulated by 2-deoxy-D-glucose in rats.
Intracisternal injection of TRH antibody blocks gastric emptying stimulated by 2-deoxy-D-glucose in rats.
复制标题
脑池内注射 TRH 抗体可阻断大鼠 2-脱氧-D-葡萄糖刺激的胃排空。
DOI：
10.1016/0006-8993(95)00005-b
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Pappas,TN
中科院分区：
文献类型：
--
作者：
Okumura,T;Taylor,IL;Ohning,G;Taché,Y;Pappas,TN
用酚红法观察了2-脱氧-D-葡萄糖（2-DG）对清醒大鼠胃排空的影响。静脉注射2-脱氧-d-葡萄糖剂量依赖性地增加胃排空率。2-DG的这种兴奋作用可被小脑下迷走神经切断术所阻断。脑池内注射促甲状腺激素释放激素（TRH）抗体阻断脑池内TRH和静脉注射2-DG诱导的胃排空增强，但不能阻断脑池内胰腺多肽诱导的胃排空刺激。腹腔注射TRH抗体没有效果。这些结果表明，内源性TRH在大脑中参与迷走神经依赖性刺激胃排空的2-DG。
We evaluated the effect of 2-deoxy-d-glucose (2-DG) on gastric emptying of a non nutrient solution in conscious rats using a Phenol red method. Intravenous injection of 2-deoxy-d-glucose dose-dependently increased the rate of gastric emptying. This stimulatory action of 2-DG was abolished by subdiaphragmatic vagotomy. Intracisternal injection of thyrotropin-releasing hormone (TRH) antibody blocked intracisternal TRH and intravenous 2-DG-induced enhancement of gastric empyting but not the stimulation of gastric emptying induced by intracisternal pancreatic polypeptide. The TRH antibody injected intraperitoneally had no effect. These results suggest that endogenous TRH in the brain is involved in vagal-dependent stimulation of gastric emptying by 2-DG.