A flyover style microfluidic chip for highly purified magnetic cell separation
A flyover style microfluidic chip for highly purified magnetic cell separation
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DOI：
10.1016/j.bios.2018.12.058
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发表时间：
2019-03-15
影响因子：
12.6
通讯作者：
Ding, Xianting
中科院分区：
文献类型：
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作者：
Lin, Shujing;Zhi, Xiao;Ding, Xianting
从外周血中分离的白细胞（wbc）已被证实是癌症诊断、治疗和预后的重要生物标志物。然而，即使利用当前的微流控技术，获得高纯度的白细胞仍然面临挑战。考虑到临床磁活化细胞分选（MACS）方法的通用性，微流控芯片结合磁细胞分离技术的新发展可能为高纯度白细胞分选和广泛的临床应用提供一种有吸引力的方法。在这里，我们提出了一种立交桥式微流控芯片，它被精心地嵌入在连续流中的两级磁分离中，用于wbc的分选。免疫磁性微纳米粒子（IMNPs）标记的白细胞（WBC@IMNPs）经横向磁力和纵向磁力依次分离，在20 μ L min（-1）的流速下，最终分离的白细胞纯度可达93 +/- 1.67%。白细胞活力达97.5 - 1.8%。因此，这种新型的立立桥式磁分离微流控芯片作为一种高纯度白细胞分选的前沿方法已经被成功证明，并且很容易推广到其他类型的细胞分选中，在生物医学领域具有很大的应用潜力。
White blood cells (WBCs) isolated from peripheral blood have been verified as important biomarkers for the diagnosis, treatment and prognosis of cancer. However, it's still under challenge to acquire high-purity WBCs, even by taking advantage of current microfluidic technology. Considering the universality of clinical magnetic activated cell sorting (MACS) method, new developments on microfluidic chip in combination of magnetic cells separation technologies may provide a fascinating approach for high-purity WBCs sorting and widely clinical application. Here, we present a flyover style microfluidic chip which has been elaborately embedded with two stage magnetic separation in continuous flow for WBCs sorting. Immunomagnetic micro/nano-particles (IMNPs) labeled WBC (WBC@IMNPs) were sequentially separated by a lateral magnetic force and a vertical magnetic force, and the final separation purity of WBCs reached up to 93 +/- 1.67% at a flow rate of 20 mu L min(-1). Furthermore, the WBCs viability was up to 97.5 1.8%. Consequently, this novel flyover style microfluidicchip with magnetic separation technology has been successfully demonstrated as cut-in-edge method for high purity WBCs sorting, and obviously it's easy to extend for other types of cells sorting under great potential application in biomedical fields.