Operation of a two-phase partitioning bioreactor for the oxidation of anthracene by the enzyme manganese peroxidase
Operation of a two-phase partitioning bioreactor for the oxidation of anthracene by the enzyme manganese peroxidase
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DOI：
10.1016/j.chemosphere.2006.07.009
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Lema, J. M.
中科院分区：
文献类型：
--
作者：
Eibes, G.;Moreira, M. T.;Lema, J. M.
研究了一种两相分配生物反应器(TPPB)处理难溶化合物菲的潜力，该酶来自真菌BJerkandera sp.BOS55。有机硅油作为不相容的溶剂，其中含有高浓度的菲。考虑了可能直接影响MNP催化循环的因素(H_2O_2和丙二酸的浓度)和影响菲在有机相和水相之间的传质的因素(溶剂和搅拌速度)，对氧化工艺进行了优化。主要目标是提高效率，即最大限度地提高每单位使用的酶所氧化的蒽量。在56h内，TPPB对菲的氧化几乎完全，转化率为1.8mg·L(-1)·h(-1)，表明该催化剂可用于难溶化合物的去除。(C)2006爱思唯尔有限公司。保留所有权利。
A study was conducted to determine the potential of a two-phase partitioning bioreactor (TPPB) for the treatment of a poorly soluble compound, anthracene, by the enzyme manganese peroxidase (MnP) from the fungus Bjerkandera sp. BOS55. Silicone oil was used as the immiscible solvent, which contained anthracene at high concentrations. The optimization of the oxidation process was conducted taking into account the factors which may directly affect the MnP catalytic cycle (the concentration of H2O2 and malonic acid) and those that affect the mass transfer of anthracene between the organic and the aqueous phase (solvent and agitation speed). The main objective was carried out in terms of improved efficiency, i.e., maximizing the anthracene oxidized per unit of enzyme used. The TPPB reached nearly complete oxidation of anthracene at a conversion rate of 1.8 mg l(-1) h(-1) in 56 h, which suggests the application of enzymatic TPPBs for the removal of poorly soluble compounds. (c) 2006 Elsevier Ltd. All rights reserved.