Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy.
Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy.
复制标题
DOI：
10.1038/nprot.2012.144
复制
发表时间：
2013-01
期刊：
影响因子：
14.8
通讯作者：
Bonnin A
中科院分区：
文献类型：
--
作者：
Goeden N;Bonnin A
离体灌注系统为研究器官对各种环境操作的急性生理反应提供了可靠的、可重复的方法。与体外培养系统不同，离体灌注器官的细胞组织、区室化和三维结构得以保持。这些特定的参数对于胎盘的正常生理功能是至关重要的，胎盘通过经胎盘交换、营养合成和代谢、生长因子促进以及母体和胎儿衍生分子的调节来支持胎儿生长。这里所述的灌注系统可以在4-5小时内完成，不仅在整个正常妊娠期，而且在各种单独的或组合的遗传和环境扰动损害胎盘功能之后，都可以进行从头合成和代谢以及材料穿过活小鼠胎盘的转运的综合生理学研究。
Ex vivo perfusion systems offer a reliable, reproducible method for studying acute physiological responses of an organ to various environmental manipulations. unlike in vitro culture systems, the cellular organization, compartmentalization and three-dimensional structure of ex vivo–perfused organs are maintained. these particular parameters are crucial for the normal physiological function of the placenta, which supports fetal growth through transplacental exchange, nutritional synthesis and metabolism, growth factor promotion and regulation of both maternally and fetally derived molecules. the perfusion system described here, which can be completed in 4–5 h, allows for integrated, physiological studies of de novo synthesis and metabolism and transport of materials across the live mouse placenta, not only throughout a normal gestation period but also following a variety of individual or combined genetic and environmental perturbations compromising placental function.