DNA-SEQUENCE DETERMINANTS OF CAP-INDUCED BENDING AND PROTEIN-BINDING AFFINITY
DNA-SEQUENCE DETERMINANTS OF CAP-INDUCED BENDING AND PROTEIN-BINDING AFFINITY
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DOI：
10.1038/333824a0
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发表时间：
1988-06-30
期刊：
影响因子：
64.8
通讯作者：
CROTHERS, DM
中科院分区：
文献类型：
--
作者：
GARTENBERG, MR;CROTHERS, DM
结合分解代谢物激活蛋白诱导的DNA弯曲的网站被确定，并显示与DNA凹槽面对蛋白质的位置相一致。在这些弯曲中心具有不同序列的DNA的可弯曲性与核小体DNA中的序列的弯曲偏好平行。各向异性DNA弯曲性显著影响调节蛋白-DNA复合物的结构和强度。
The sites of DNA bending induced by binding catabolite activator protein are identified and shown to coincide with positions where DNA grooves face the protein. The bendability of DNA with different sequences at these bend centres parallels the bending preference of the sequences in nucleosomal DNA. Anisotropic DNA bendability significantly affects the structure and strength of regulatory protein –DNA complexes.